Does it require withholding vitamin D (Vit D) supplement before checking estradiol levels?

Yes, it is generally recommended to hold DHEA (dehydroepiandrosterone) supplements before checking estradiol levels. Ideally, you should discontinue DHEA for at least 1-2 weeks prior to blood testing. DHEA is a precursor hormone that can be converted to estrogens in the body, including estradiol, through a process called aromatization. Taking DHEA supplements can artificially elevate your estradiol levels, which may lead to inaccurate test results and potentially inappropriate treatment decisions. This is particularly important if the estradiol test is being used to make decisions about hormone therapy, fertility treatments, or to investigate hormonal imbalances. If you're unable to stop taking DHEA before your test, be sure to inform your healthcare provider about your supplement use so they can interpret your results accordingly. Always consult with your healthcare provider before stopping any prescribed supplements or medications.