What is the recommended dosage and schedule for M-Spikevax (COVID-19 vaccine, mRNA-1273.214)?

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Last updated: November 10, 2025View editorial policy

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M-Spikevax (mRNA-1273.214) Dosage and Schedule

M-Spikevax (mRNA-1273.214) is administered as a single 50 μg booster dose at least 2 months after completion of any FDA-approved or FDA-authorized COVID-19 primary series or previous monovalent booster dose for adults aged ≥18 years. 1

Primary Series vs. Booster Dosing

M-Spikevax specifically refers to the bivalent formulation (mRNA-1273.214) containing equal amounts of spike mRNA from the ancestral SARS-CoV-2 strain and Omicron BA.4/BA.5 subvariants. 1 This is distinct from the original monovalent Spikevax (mRNA-1273) used for primary series vaccination. 1

  • For primary series: The original monovalent Spikevax is used as a 2-dose series (or 3-dose for immunocompromised individuals), not M-Spikevax. 1
  • For booster doses: M-Spikevax (bivalent BA.4/BA.5) is administered as a single 50 μg dose. 1

Timing Recommendations

  • Minimum interval: Administer ≥2 months after the last dose of any COVID-19 vaccine (primary series or monovalent booster). 1
  • Optional delay after infection: Individuals who recently had SARS-CoV-2 infection may consider delaying the booster by 3 months from symptom onset or positive test, though this is not required. 1

Special Populations

Immunocompromised Patients

  • Immunocompromised adults follow the same booster dosing schedule (single 50 μg dose ≥2 months after last vaccine), but may have received a 3-dose primary series initially. 1
  • These patients should also be considered for preexposure prophylaxis with monoclonal antibodies to supplement vaccine protection. 1

Patients on Immunomodulatory Therapy

For patients on certain immunosuppressive medications, timing adjustments may optimize vaccine response:

  • Methotrexate: Consider holding for 1 week after each vaccine dose if disease activity permits. 1
  • JAK inhibitors: Consider holding for 1 week after each vaccine dose regardless of disease activity. 1
  • Subcutaneous abatacept: Hold for 1 week before and 1 week after the first dose only (total 2 weeks), but no adjustment needed for subsequent doses. 1
  • Rituximab: Time administration 2-4 weeks after the final vaccine dose if disease control permits. 1

Important Clinical Considerations

Common pitfall: Do not confuse M-Spikevax (bivalent booster) with the original Spikevax (monovalent primary series vaccine). The bivalent formulation is only authorized for booster doses, not primary series. 1

Coadministration: M-Spikevax can be administered simultaneously with other vaccines, including influenza vaccine, without any required waiting period between doses. 2

Reactogenicity: Solicited adverse reactions occur in approximately 90% of recipients, similar to the original mRNA-1273 vaccine, with no new safety concerns identified. 3

Immunogenicity Data

The bivalent BA.4/BA.5 formulation demonstrates superior neutralizing antibody responses against Omicron subvariants compared to monovalent boosters, with GMC ratios of 1.53-1.68 against BA.1 variant. 3 Cross-neutralization extends to more recent variants including XBB.1.5, EG.5.1, BA.2.86, and JN.1. 4

Real-world effectiveness data from Nordic countries shows 67.8% (95% CI 63.1-72.5%) comparative vaccine effectiveness against COVID-19 hospitalization and 69.8% (95% CI 52.8-86.8%) against COVID-19-related death at 3 months post-vaccination in adults ≥50 years. 5

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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