What is the normal Amniotic Fluid Index (AFI) range at 27 weeks of gestation?

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Last updated: November 12, 2025View editorial policy

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Normal AFI at 27 Weeks Gestation

At 27 weeks gestation, the normal Amniotic Fluid Index (AFI) range is 5-24 cm, with values below 5 cm indicating oligohydramnios and above 24 cm indicating polyhydramnios. 1

Gestational Age-Specific Considerations

While the general normal range of 5-24 cm applies across gestation, important nuances exist at 27 weeks:

  • Before 37 weeks gestation, an AFI of 8 cm or greater is commonly used to define normal amniotic fluid, which is more stringent than the lower threshold of 5 cm used at term 1

  • Research data from the NICHD Fetal Growth Studies shows that AFI values peak around 26 weeks gestation, making 27 weeks a time of relatively high amniotic fluid volume 2

  • At 27 weeks specifically, the 3rd percentile AFI is approximately 10.1 cm and the 97th percentile is in the range of 24-25 cm, suggesting that single cutoffs may not capture the full physiologic variation 2

Clinical Interpretation at 27 Weeks

The volume of fluid per centimeter of AFI changes throughout pregnancy, which affects interpretation:

  • At 20 weeks, each cm of AFI represents approximately 25 mL of fluid, but this increases to about 40 mL per cm by 30 weeks—a 61% increment 3

  • This means an AFI of 18 cm at 27 weeks represents substantially more actual fluid volume than the same AFI measurement at earlier gestations 3

Diagnostic Thresholds

When evaluating AFI at 27 weeks:

  • AFI < 5 cm defines oligohydramnios and is independently associated with increased stillbirth risk (odds ratio 2.6) 1, 4

  • AFI > 24 cm or >95th percentile defines polyhydramnios and is associated with increased perinatal mortality (odds ratio 5.8) 1

  • Consider using Maximum Vertical Pocket (MVP) ≥ 2 cm as an alternative measurement, as it may result in fewer false diagnoses of oligohydramnios and unnecessary interventions compared to AFI 1, 4

Common Pitfalls to Avoid

  • Do not apply term pregnancy thresholds (AFI ≥ 5 cm) to preterm gestations like 27 weeks—the threshold of 8 cm is more appropriate before 37 weeks 1

  • Avoid relying solely on AFI for clinical decisions without considering other fetal surveillance parameters, as isolated AFI abnormalities may lead to overdiagnosis 4

  • Be aware that racial/ethnic differences exist in AFI measurements, though these differences are most prominent after 35 weeks and may not be clinically significant at 27 weeks 2

References

Guideline

Amniotic Fluid Index Measurement and Clinical Significance

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Guideline

Management of Oligohydramnios in Pregnancy

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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