What type of antibiotic is Levofloxacin (fluoroquinolone)?

Medical Advisory BoardAll articles are reviewed for accuracy by our Medical Advisory Board
Educational purpose only • Exercise caution as content is pending human review
Article Review Status
Submitted
Under Review
Approved

Last updated: November 12, 2025View editorial policy

Personalize

Help us tailor your experience

Which best describes you? Your choice helps us use language that's most understandable for you.

Levofloxacin Classification

Levofloxacin is a fluoroquinolone antibiotic, specifically the L-isomer of the racemic drug ofloxacin. 1

Mechanism of Action

Fluoroquinolones exert their bactericidal activity by binding to bacterial DNA gyrase and topoisomerase IV, which impedes the formation of supercoiled DNA, inhibits the relaxation of supercoiled DNA, and promotes double-strand DNA breakage. 2, 1

Antimicrobial Spectrum

Levofloxacin demonstrates broad-spectrum activity against:

  • Gram-positive organisms including Streptococcus pneumoniae (both penicillin-susceptible and penicillin-resistant strains) 2, 3
  • Gram-negative organisms including Haemophilus influenzae and Moraxella catarrhalis 2
  • Atypical respiratory pathogens including Mycoplasma pneumoniae, Chlamydophila pneumoniae, and Legionella pneumophila 2, 4
  • Mycobacteria including Mycobacterium tuberculosis 2

Comparative Activity Within Fluoroquinolones

Levofloxacin has enhanced activity against Gram-positive organisms compared to ciprofloxacin, particularly against S. pneumoniae, though it is less active than newer fluoroquinolones such as gatifloxacin and moxifloxacin. 2, 3 Ciprofloxacin has an AUC-to-MIC ratio against S. pneumoniae of only 10-20, whereas levofloxacin and other "respiratory fluoroquinolones" achieve the target AUC-to-MIC ratio of approximately 25-30. 2

Cross-resistance has been demonstrated among ciprofloxacin, ofloxacin, and levofloxacin, and is presumably a class effect of fluoroquinolones. 2

Clinical Positioning

Among the fluoroquinolones, levofloxacin, moxifloxacin, and gatifloxacin have the most activity against M. tuberculosis, with levofloxacin being the preferred oral agent for treating drug-resistant tuberculosis based on cumulative experience suggesting a good safety profile with long-term use. 2

Levofloxacin and moxifloxacin are the only currently UK-licensed fluoroquinolones with enhanced activity against S. pneumoniae, in addition to coverage for Staphylococcus aureus. 2

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

Have a follow-up question?

Our Medical A.I. is used by practicing medical doctors at top research institutions around the world. Ask any follow up question and get world-class guideline-backed answers instantly.