What is the recommended protocol for ocular disinfection in ophthalmic surgery?

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Last updated: November 12, 2025View editorial policy

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Ocular Disinfection Protocol for Ophthalmic Surgery

Perform preoperative ocular disinfection using povidone-iodine with 10% solution on periocular skin and 5% solution on the ocular surface (cornea, conjunctiva, and fornices), followed by thorough irrigation with sterile saline. 1, 2

Preoperative Preparation

Periocular Skin Disinfection

  • Apply 10% povidone-iodine to the periocular region (lids, brow, and cheek) as this concentration significantly reduces endophthalmitis risk compared to 5% on skin 3
  • Allow adequate contact time for bactericidal effect 2

Ocular Surface Disinfection

  • Instill 5% povidone-iodine solution directly onto the ocular surface (cornea, conjunctiva, and palpebral fornices) 1, 2, 4
  • This concentration reduces conjunctival bacterial flora from 66% to 30% positive cultures compared to saline alone 4
  • Flush the eye thoroughly with sterile saline solution after application to remove residual povidone-iodine and prevent toxicity 2

Critical Timing Considerations

  • Apply povidone-iodine immediately before surgery within the preoperative preparation 5
  • For enhanced protection, consider 3-day preoperative course of broad-spectrum topical antibiotics (such as fluoroquinolones) followed by povidone-iodine application on the day of surgery 5

Alternative Dosing Strategy for Enhanced Sterility

For high-risk cases or prolonged procedures, consider repetitive application of dilute (0.25%) povidone-iodine every 30 seconds throughout surgery rather than single 5% application 6, 7

Rationale for Dilute Repetitive Dosing

  • Dilute concentrations (0.05%-1.0%) kill bacteria more rapidly than conventional 5%-10% concentrations due to greater availability of free diatomic iodine 6
  • Repetitive dilute application maintains transient ocular surface sterility throughout the procedure 7
  • Lower toxicity profile compared to higher concentrations 6, 7
  • Effective against multidrug-resistant bacteria, fungi, and novel pathogens without inducing resistance 7

Instrument and Equipment Sterilization

Ensure strict sterilization of all surgical instruments and improve disinfection methods to avoid residual disinfectant contamination 1

Key Points

  • Carefully examine and clean the operative eye before disinfection 1
  • Avoid residual disinfectant which can cause postoperative inflammation 1
  • Minimize surgical duration and light exposure to reduce ocular surface stress 1

Common Pitfalls to Avoid

  • Never use povidone-iodine for intraocular injection or irrigation - it is for external use only 2
  • Do not use on patients with known iodine sensitivity 2
  • Avoid inadequate flushing after povidone-iodine application, which increases toxicity risk 2
  • Do not substitute with less effective alternatives like hypochlorous acid, which has significantly reduced efficacy against endophthalmitis isolates 7
  • Avoid using 5% povidone-iodine on periocular skin when 10% is available, as this increases infection risk 3

Adjunctive Measures

  • Consider periocular administration or intraocular injection of antibiotics and/or steroids immediately before the end of surgery 1
  • Use viscosurgical devices during surgery to protect ocular tissues 1
  • Minimize thermal energy from phacoemulsification devices 1
  • Avoid large corneal incisions and aspirating speculums 1

References

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Research

Risk of endophthalmitis after cataract surgery using different protocols for povidone- iodine preoperative disinfection.

Journal of ocular pharmacology and therapeutics : the official journal of the Association for Ocular Pharmacology and Therapeutics, 2006

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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