What are the recommendations for the Japanese Encephalitis (JE) vaccine, including administration and side effects?

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Last updated: November 14, 2025View editorial policy

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Japanese Encephalitis Vaccine Recommendations

The Japanese Encephalitis vaccine (JE-VC) is recommended for travelers with longer-term stays (≥1 month) in endemic areas, those moving to endemic countries, or frequent travelers to these regions, administered as a 2-dose intramuscular series that must be completed at least 1 week before potential exposure. 1

Who Should Receive the Vaccine

Definite Indications

  • Moving to a JE-endemic country to take up residence 1
  • Longer-term travel (≥1 month) to JE-endemic areas 1
  • Frequent travel to JE-endemic areas 1
  • All laboratory workers with potential exposure to JE viruses (except those working only with SA14-14-2 vaccine virus) 1

Consider Vaccination For

  • Shorter-term travel (<1 month) with increased risk based on rural location, transmission season, outdoor activities (especially sunset through dawn), and accommodations without air conditioning, screens, or bed nets 1
  • Travel to JE-endemic areas with uncertain duration, destination, or activities 1

Do NOT Vaccinate

  • Very low-risk travelers with shorter-term travel limited to urban areas OR travel outside well-defined JE virus transmission seasons 1

Primary Vaccination Schedule

Age-Specific Dosing (Intramuscular Administration)

  • Ages 2-35 months: 2 doses of 0.25 mL each on days 0 and 28 1
  • Ages 3-17 years: 2 doses of 0.5 mL each on days 0 and 28 1
  • Ages 18-65 years: 2 doses of 0.5 mL each on days 0 and 7-28 (accelerated schedule available) 1
  • Ages >65 years: 2 doses of 0.5 mL each on days 0 and 28 1

Critical Timing

  • Complete the 2-dose series at least 1 week before potential JE virus exposure 1

Vaccine Preparation and Administration

Preparation

  • Shake the syringe well to obtain a white, opaque, homogeneous suspension (vaccine may appear as clear liquid with white precipitate during storage) 1
  • For 0.25 mL doses: Expel and discard half the volume from the 0.5 mL prefilled syringe by pushing the plunger stopper to the edge of the red line 1
  • Store at 35°F-46°F (2°C-8°C), do not freeze, protect from light 1

Administration Technique

  • Administer intramuscularly at 90-degree angle 2
  • Preferred sites: Deltoid muscle for adults and older children; anterolateral thigh for infants and younger children 2
  • Use separate syringes and different anatomical sites (>1 inch apart if possible) when administering concomitantly with other vaccines 1

Booster Doses

  • A single booster dose (0.25 mL for ages 2-35 months; 0.5 mL for ≥3 years) may be given if ongoing risk exists and ≥1 year has elapsed since primary series 1
  • Clinical trial data show high seroprotection rates for at least 6 years after a booster dose 1
  • No U.S. recommendations exist for subsequent booster doses beyond the first 1

Contraindications and Precautions

Absolute Contraindications

  • Severe allergic reaction (anaphylaxis) after previous JE vaccine dose or any vaccine component 1
  • Known hypersensitivity to protamine sulfate (contained in JE-VC) 1

Precautions

  • Pregnancy: Usually defer vaccination due to theoretical fetal risk; however, vaccinate pregnant women traveling to high-risk areas when benefits outweigh risks 1
  • Infants <2 months: Safety and effectiveness not established 1
  • Adults ≥65 years: Lower seroprotection rates and GMT compared to younger persons, though no data support additional or early booster doses 1
  • Immunocompromised persons: May have diminished response; no specific data available 1

Not Contraindications

  • Breastfeeding: Not a contraindication or precaution 1

Adverse Events and Safety Monitoring

Common Side Effects

  • Approximately 50% of vaccinees experience usually mild adverse effects 3

Serious Allergic Reactions

  • Generalized urticaria and angioedema can occur from minutes to 2 weeks after vaccination 1
  • Rates of serious allergic reactions are low (1-104 per 10,000) 1

Safety Measures

  • Observe vaccinees for 30 minutes after vaccination 1
  • Warn patients about possibility of delayed urticaria and angioedema of head and airway 1
  • Advise vaccinees to remain in areas with ready access to medical care for 10 days after vaccination 1
  • Have epinephrine and equipment to treat anaphylaxis available 1

Reporting

  • Report all clinically significant adverse events to VAERS (https://vaers.hhs.gov or 800-822-7967), even if causal relation uncertain 1

Concomitant Vaccine Administration

  • JE-VC can be administered concomitantly with hepatitis A vaccine without interference 1
  • JE-VC can be administered concomitantly with rabies vaccine with noninferiority established 1
  • Use separate syringes and different anatomical sites (>1 inch apart if possible) for concomitant administration 1

Risk Assessment Factors

Geographic Risk

  • Highest risk in rural or agricultural areas with flooded rice fields, marshes, and stagnant water collections 1
  • Cases reported among travelers to coastal areas or resorts adjacent to rural/rice-growing areas 1
  • Risk varies within countries and from year to year 1

Temporal Risk

  • Transmission occurs seasonally in some areas, year-round in others 1
  • Mosquitoes feed most often outdoors from sunset through dawn 1

Activity-Related Risk

  • Outdoor activities, especially evening through dawn, increase risk 1
  • Accommodations without air conditioning, screens, or bed nets increase mosquito exposure 1

References

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Guideline

Needle Selection and Administration Technique for Boostrix (Tdap) 0.5ml

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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