When should a workup for neurofibromatosis (NF) be considered in individuals with a large single cafe-au-lait spot?

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Last updated: November 18, 2025View editorial policy

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When to Work Up for Neurofibromatosis with a Large Single Café-au-Lait Spot

A single large café-au-lait macule alone does not warrant workup for neurofibromatosis type 1 (NF1), as the NIH diagnostic criteria require ≥6 café-au-lait macules (≥5mm prepubertal or ≥15mm postpubertal) to meet even one criterion for NF1. 1

Key Diagnostic Threshold

  • The presence of ≥6 café-au-lait macules meeting size criteria is required as one of the NIH diagnostic criteria for NF1, not a single spot regardless of size 1
  • Document the exact number and size of all café-au-lait spots present, as this is crucial for determining if diagnostic criteria are met 1

When to Pursue Further Evaluation

Initiate workup if the patient has a single large café-au-lait spot PLUS any of the following:

  • Axillary or inguinal freckling (Crowe's sign), which is highly specific for NF1 1
  • Family history of NF1 in a first-degree relative 1
  • Cutaneous or subcutaneous neurofibromas on examination 1
  • Dysmorphic facial features, congenital heart defects, short stature, or cryptorchidism, suggesting RASopathies (Noonan, Costello, or CBL syndrome) rather than isolated café-au-lait spots 1
  • Developmental delays, hypotonia, or leukemia diagnosed at age <18, which warrant genetics referral 1
  • Lisch nodules on slit-lamp examination 1

Evidence on Isolated Café-au-Lait Macules

  • Among children presenting with isolated café-au-lait macules, 19.5% to 57.1% do not ultimately receive an NF1 diagnosis after follow-up or genetic testing 2
  • Children older than 29 months with fewer than 6 café-au-lait macules or atypical macules have only a 0.9% risk for constitutional NF1 3
  • In contrast, children younger than 29 months with ≥6 café-au-lait macules have an 80.4% risk for NF1 3

Referral Criteria

Refer to genetics when the patient meets ≥2 NIH diagnostic criteria for NF1:

  • This includes ≥6 café-au-lait macules PLUS one additional criterion (freckling, neurofibromas, Lisch nodules, optic glioma, bone dysplasia, or family history) 1
  • Genetic testing can establish diagnosis and guide surveillance, though a single café-au-lait spot alone does not meet referral threshold 1

Important Differential Diagnosis

  • Legius syndrome (SPRED1 mutations) mimics NF1 with café-au-lait macules and freckling but lacks neurofibromas and optic gliomas—distinguishing this is critical as surveillance requirements differ markedly 4, 1
  • Familial multiple café-au-lait spots can occur as an autosomal dominant condition without NF1, even with axillary freckling or Lisch nodules in some families 5

Common Pitfalls to Avoid

  • Do not diagnose NF1 based on a single café-au-lait spot, regardless of size—this leads to unnecessary anxiety and inappropriate surveillance 1
  • Do not confuse Legius syndrome with NF1—Legius patients lack tumor risks and require different management 1
  • Do not delay referral in children with café-au-lait macules plus developmental delays, hypotonia, or leukemia, as these suggest syndromic diagnoses requiring specialized care 1

References

Guideline

Management and Evaluation of Significant Café au Lait Spots

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Research

Familial multiple cafe au lait spots.

Archives of dermatology, 1994

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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