Does rifampicin (RIF) interact with ethambutol (EMB), pyrazinamide (PZA), isoniazid (INH), streptomycin (SM), or moxifloxacin (MXF)?

Medical Advisory BoardAll articles are reviewed for accuracy by our Medical Advisory Board
Educational purpose only • Exercise caution as content is pending human review
Article Review Status
Submitted
Under Review
Approved

Last updated: November 18, 2025View editorial policy

Personalize

Help us tailor your experience

Which best describes you? Your choice helps us use language that's most understandable for you.

Rifampin Drug Interactions with First-Line TB Medications

Rifampin does NOT have clinically significant drug interactions with ethambutol, pyrazinamide, isoniazid, streptomycin, or moxifloxacin when used together for tuberculosis treatment. 1

Direct Evidence from Guidelines

The American Thoracic Society/CDC/IDSA explicitly states that "no contraindication exists for the use of NRTIs, NNRTIs, and protease inhibitors with isoniazid, pyrazinamide, ethambutol, or streptomycin. These first-line antituberculosis medications, in contrast to the rifamycins, are not CYP450 inducers." 1 This confirms these TB drugs do not interact with rifampin through the cytochrome P450 system, which is rifampin's primary mechanism of drug interactions. 1

Standard Combination Regimens

Multiple guidelines recommend using these drugs together without dose adjustments:

  • The FDA-approved regimen consists of rifampin, isoniazid, and pyrazinamide for 2 months, with either streptomycin or ethambutol added as a fourth drug. 2

  • This is followed by rifampin and isoniazid for at least 4 additional months. 2

  • All five drugs (rifampin, isoniazid, pyrazinamide, ethambutol, streptomycin) can be administered together in the initial phase without concern for drug-drug interactions between them. 1, 3

Intermittent Dosing Compatibility

The drugs maintain their lack of interaction even with intermittent dosing schedules:

  • Twice-weekly or three-times-weekly administration of rifampin with isoniazid, pyrazinamide, ethambutol, and streptomycin has been proven highly effective. 1, 4

  • A study of 833 patients showed bacteriological relapse rates of 2% or less for all pyrazinamide-containing regimens given three times weekly. 4

Moxifloxacin Considerations

Moxifloxacin (a fluoroquinolone) can be safely added to rifampin-containing regimens for drug-resistant TB or extensive disease. 1

  • Fluoroquinolones are recommended to strengthen regimens for patients with more extensive disease or isoniazid resistance. 1

  • The interaction concern with fluoroquinolones relates to absorption interference by metal cations, not rifampin. 5, 6

Hepatotoxicity: Additive, Not Interactive

The primary concern when combining these drugs is additive hepatotoxicity, not drug-drug interactions:

  • Rifampin may enhance isoniazid hepatotoxicity through enzyme induction, causing early transaminase elevation (within 15 days). 7

  • The combination of isoniazid, rifampin, and pyrazinamide has a clinical hepatitis rate of 2.7%, compared to 0.6% for isoniazid alone. 1

  • This represents additive toxicity rather than a pharmacokinetic interaction that would require dose adjustment. 7

Monitoring Requirements

Monitor serum transaminases twice weekly during the first 2 weeks, every 2 weeks during the first 2 months, then monthly thereafter. 7

  • Stop all three drugs (isoniazid, rifampin, pyrazinamide) if transaminases exceed 3 times the upper limit of normal. 7

  • After normalization, reintroduce isoniazid at low dose without rifampin initially; do not reintroduce pyrazinamide due to poor prognosis of pyrazinamide-induced hepatitis. 7

Important Caveat

Rifampin's major drug interactions occur with OTHER drug classes (antiretrovirals, oral contraceptives, anticoagulants, immunosuppressants) through CYP450 induction, NOT with standard TB medications. 1, 8, 9

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

Have a follow-up question?

Our Medical A.I. is used by practicing medical doctors at top research institutions around the world. Ask any follow up question and get world-class guideline-backed answers instantly.