What are the first-line and second-line treatment options for Helicobacter pylori (H pylori) infection?

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Last updated: November 19, 2025View editorial policy

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H. pylori: Important Points for Exam

First-Line Treatment Regimens

Bismuth quadruple therapy for 14 days is the preferred first-line treatment for H. pylori eradication, particularly in areas with high clarithromycin resistance (≥15%). 1, 2

Bismuth Quadruple Therapy Components:

  • PPI twice daily (standard or double dose) 1
  • Bismuth subsalicylate 262 mg, 2 tablets OR bismuth subcitrate 120 mg, 1 tablet four times daily 1
  • Metronidazole 500 mg three to four times daily 1, 2
  • Tetracycline 500 mg four times daily 1, 2
  • Duration: 14 days (preferred over 10 days for ~5% improvement in eradication) 1, 2

This regimen achieves 80-90% eradication rates even against metronidazole-resistant strains due to bismuth's synergistic effect. 3

Alternative First-Line: Concomitant Non-Bismuth Quadruple Therapy

When bismuth is unavailable: 1, 2

  • PPI twice daily
  • Amoxicillin 1000 mg twice daily
  • Clarithromycin 500 mg twice daily
  • Metronidazole 500 mg twice daily
  • Duration: 14 days

Critical caveat: Only use in areas with clarithromycin resistance <15%, as resistance >15% makes this regimen unacceptably ineffective. 1, 3

Outdated First-Line: Standard Triple Therapy

Standard triple therapy (PPI + clarithromycin + amoxicillin) should be abandoned when regional clarithromycin resistance exceeds 15-20%. 1, 3 This threshold has been surpassed in most of North America and Europe, where resistance now exceeds 20%. 3

Second-Line Treatment After First Failure

All guidelines agree: avoid re-using antibiotics that failed previously, especially clarithromycin and levofloxacin, where resistance develops rapidly after exposure. 1

Second-Line Options:

  1. Bismuth quadruple therapy (if not used first-line) for 14 days 1, 2

  2. Levofloxacin triple therapy (if bismuth/metronidazole used first-line): 1, 2

    • PPI twice daily
    • Amoxicillin 1000 mg twice daily
    • Levofloxacin 500 mg once daily OR 250 mg twice daily
    • Duration: 14 days

Important warning: Levofloxacin resistance is rapidly increasing (11-30% primary, 19-30% secondary resistance), so susceptibility testing should be strongly considered before using this regimen. 1, 3

Third-Line and Salvage Therapy

After two failed eradication attempts, antimicrobial susceptibility testing should guide further treatment whenever possible. 1, 4

Salvage Options When Testing Unavailable:

  • High-dose dual therapy: PPI (high dose twice daily) + amoxicillin 1000 mg three times daily for 14 days 1, 3
  • Rifabutin triple therapy: PPI + amoxicillin + rifabutin (restricted to multiple failures) 1, 3
  • Use antibiotics not previously used: amoxicillin, tetracycline, bismuth, or furazolidone (resistance to these remains rare) 1, 4

Special Populations

Penicillin Allergy:

  • First choice: Bismuth quadruple therapy (contains tetracycline, not amoxicillin) 1
  • Alternative in low clarithromycin resistance areas: PPI + clarithromycin + metronidazole 1
  • Consider penicillin allergy testing to enable amoxicillin use, as amoxicillin resistance remains rare. 3

Pediatric Patients:

  • Cannot use: Fluoroquinolones or tetracyclines 1, 2
  • First-line options: PPI + amoxicillin + clarithromycin OR PPI + amoxicillin + metronidazole OR bismuth + amoxicillin + metronidazole 1
  • Susceptibility testing for clarithromycin is recommended before use. 1

Critical Treatment Optimization Factors

PPI Dosing:

High-dose PPI (twice daily) is mandatory—standard once-daily dosing is inadequate. 2, 5 This increases eradication efficacy by 6-12% by reducing gastric acidity and enhancing antibiotic activity. 5, 3

Standard PPI doses: 1

  • Esomeprazole 20-40 mg
  • Omeprazole 20-40 mg
  • Lansoprazole 30 mg
  • Pantoprazole 40 mg
  • Rabeprazole 20 mg

Treatment Duration:

14-day regimens are superior to 7-10 day regimens, improving eradication by approximately 5%. 1, 2, 5

Adjunctive Probiotics:

Probiotics reduce antibiotic-related side effects (especially diarrhea, which occurs in 21-41% of patients) and may increase eradication rates by 5-10%. 1, 2, 4

Verification of Eradication

Confirm eradication with urea breath test OR monoclonal stool antigen test at least 4 weeks after completion of therapy and at least 2 weeks after PPI discontinuation. 2, 5, 3

Never use serology to confirm eradication—antibodies persist long after successful treatment. 2, 5

Antibiotic Resistance Patterns (Critical for Exam)

Global Resistance Rates:

  • Clarithromycin: 10-34% (primary), 15-67% (secondary)—increased from 9% (1998) to 17.6% (2008-2009) 5, 3
  • Levofloxacin: 11-30% (primary), 19-30% (secondary) 3
  • Metronidazole: 23-56% (primary), 30-65% (secondary) 3
  • Amoxicillin: 1-5% (remains low) 3
  • Tetracycline: 1-5% (remains low) 3
  • Bismuth: No resistance described 1

When H. pylori is clarithromycin-resistant, eradication rates drop from 90% to approximately 20%. 3

Common Pitfalls to Avoid

  1. Never assume low clarithromycin resistance without local surveillance data—most regions now have high resistance. 3

  2. Never repeat clarithromycin if patient has prior macrolide exposure for any indication—cross-resistance is universal within the macrolide family. 3

  3. Never use standard-dose PPI once daily—always use twice-daily dosing. 3

  4. Never use levofloxacin empirically as first-line therapy due to rapidly rising fluoroquinolone resistance. 3

  5. Metronidazole can be re-used with bismuth (unlike clarithromycin/levofloxacin) because bismuth's synergistic effect overcomes in vitro resistance. 1

  6. Amoxicillin and tetracycline can be re-used because resistance to these agents remains rare. 1

Risk Factors for Treatment Failure

  • Smoking: Odds ratio 1.95 for eradication failure 3
  • High BMI/obesity: Lower drug concentrations at gastric mucosal level 3
  • Poor compliance: >10% of patients are poor compliers 3
  • Prior antibiotic exposure: Especially macrolides and fluoroquinolones 1, 3

FDA-Approved H. pylori Regimens

Triple Therapy (Adults):

Amoxicillin 1 gram + clarithromycin 500 mg + lansoprazole 30 mg, all twice daily for 14 days 6

Dual Therapy (Adults with clarithromycin allergy/intolerance):

Amoxicillin 1 gram + lansoprazole 30 mg, three times daily for 14 days 6

References

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Guideline

Helicobacter Pylori Eradication Regimens

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Guideline

Helicobacter Pylori Infection Treatment Guidelines

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Research

Management of Helicobacter pylori infection.

JGH open : an open access journal of gastroenterology and hepatology, 2023

Guideline

H. pylori-Related Pain Treatment

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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