What is the most likely diagnosis for a patient with an abdominal mass and a positive anal canal biopsy for human papillomavirus (HPV)?

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Last updated: November 23, 2025View editorial policy

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Anal Squamous Cell Carcinoma (SCC)

The most likely diagnosis is D. Anal SCC, given the presence of HPV on biopsy and a palpable mass in the anal canal. 1

Rationale Based on HPV Association

HPV is the causative agent in 80-85% of anal squamous cell carcinomas, making this the strongest association among the answer choices. 1, 2 Specifically, HPV types 16 and 18 are most commonly implicated in anal SCC and its precursor lesion, anal intraepithelial neoplasia (AIN). 1, 2

  • HPV is detected in approximately 90% of anal squamous cell cancers, making it second only to cervical cancer in the closeness of its association with this virus. 2
  • The European Society for Medical Oncology (ESMO) guidelines explicitly state that squamous cell carcinoma of the anus (SCCA) and AIN are mostly attributable to HPV infection. 1

Clinical Presentation Consistent with Anal SCC

SCCA commonly presents with a mass, which aligns with this patient's abdominal/anal mass. 1

  • According to ESMO guidelines, SCCA may present with any combination of a mass, non-healing ulcer, pain, bleeding, itching, discharge, fecal incontinence, and fistulae. 1
  • The diagnosis of anal cancer is made by biopsy-proven histology, which has been performed in this case showing HPV. 1

Why Other Options Are Less Likely

Paget's disease (Option A) is not primarily HPV-associated and typically presents as an eczematous lesion rather than a mass with HPV positivity. 1

Bowen disease (Option B) represents squamous cell carcinoma in situ of the skin, but when HPV-positive lesions occur in the anal canal with a mass, they are classified as invasive squamous cell carcinoma rather than Bowen disease. 1

Adenocarcinoma (Option C) is specifically listed in ESMO guidelines as among the differential diagnoses that must be distinguished from squamous cell carcinomas through histological confirmation. 1 Adenocarcinomas of the anal canal are not typically HPV-associated and would show glandular rather than squamous differentiation on biopsy. 1

Diagnostic Confirmation

Histological confirmation is mandatory according to ESMO guidelines, as histopathological entities other than SCCs include adenocarcinoma, melanoma, gastrointestinal stromal tumors, poorly differentiated neuroendocrine tumors, and lymphoma. 1

  • P16/HPV assessment is recommended in the diagnostic work-up of SCCA. 1
  • The presence of HPV on biopsy strongly supports the diagnosis of squamous cell carcinoma over other histologic types. 1, 2

Common Pitfall to Avoid

Do not dismiss anal bleeding or masses as hemorrhoids, as diagnosis of SCCA is often delayed because bleeding is attributed to hemorrhoids. 1, 2 Digital anorectal examination is an essential low-cost clinical tool for detection of lesions in the anal area and should be performed in all patients with anal symptoms. 1

References

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Guideline

Primary Cause and Risk Factors for Anal Cancer

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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