What is the recommended daily dose of Vitamin D3 (Cholecalciferol) for infants?

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Last updated: November 25, 2025View editorial policy

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Vitamin D3 Dosing for Infants

All infants should receive 400 IU of oral vitamin D3 daily starting at hospital discharge, regardless of whether they are breastfed or formula-fed, to prevent vitamin D deficiency and rickets. 1, 2

Standard Dosing by Age and Population

Term Infants (0-12 months)

  • 400 IU/day is the recommended dose for all healthy term infants from birth through 12 months of age 3, 1
  • This applies to both breastfed and formula-fed infants, though formula-fed infants consuming ≥28 ounces of fortified formula daily may not require additional supplementation 2
  • Supplementation should begin at hospital discharge, not delayed until the first outpatient visit 2

Preterm Infants

  • Preterm infants require 200-400 IU/day initially, with some extremely preterm infants potentially needing up to 800-1,000 IU/day 2
  • For preterm infants on parenteral nutrition, the dose range is 200-1,000 IU/day (or 80-400 IU/kg/day) 3

Evidence Supporting Higher Doses

While 400 IU/day remains the guideline-recommended dose, emerging research suggests this may be insufficient in certain populations:

  • A 2022 randomized controlled trial from a low-middle income country demonstrated that 800 IU/day reduced vitamin D insufficiency by 50% compared to 400 IU/day (24% vs 55% with insufficiency) with no toxicity 4
  • At 14 weeks, 21.3% of infants receiving 200 IU/day had deficient levels, while none receiving 400 IU/day were deficient 5
  • However, current guidelines have not yet incorporated these findings, and 400 IU/day remains the standard recommendation 1, 2, 6

Alternative Supplementation Strategy

For breastfeeding mothers who prefer an alternative approach, maternal supplementation with 6,400 IU/day of vitamin D3 can provide adequate vitamin D to the infant through breast milk 2, 6. This eliminates the need for direct infant supplementation but requires excellent maternal compliance.

Safety Thresholds

  • Upper tolerable limit for infants 0-6 months: 1,000 IU/day 1, 2
  • Upper tolerable limit for infants 7-12 months: 1,500 IU/day 1, 2
  • Vitamin D toxicity is very uncommon at recommended doses, but caution should be used with extremely concentrated commercial drops 6

Special Populations Requiring Monitoring

  • Infants with dark skin may benefit from 600 IU/day rather than 400 IU/day 7
  • Infants with malabsorption conditions, liver disease, kidney disease, or on long-term parenteral nutrition require periodic monitoring of 25(OH)D levels and potentially higher supplementation 1, 2
  • Target serum 25(OH)D level is >20 ng/mL (50 nmol/L) 1

Common Pitfalls to Avoid

  • Do not delay supplementation until the first outpatient visit; begin at hospital discharge 2
  • Do not assume formula-fed infants are automatically sufficient; most infants don't consume 28 ounces of formula daily until 2 months of age 6
  • Avoid using extremely concentrated vitamin D drops that could lead to accidental overdosing 6
  • Do not use active vitamin D analogs (calcitriol) to treat nutritional vitamin D deficiency 1

References

Guideline

Treatment for Vitamin D Deficiency in Children

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Guideline

Vitamin D Supplementation for Newborns

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Research

Vitamin D in Preterm and Full-Term Infants.

Annals of nutrition & metabolism, 2020

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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