Does Botroclot contain gem coagulase or another thrombin-like enzyme, such as batroxobin?

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Botroclot Contains Batroxobin, Not Gem Coagulase

Botroclot is a marketed preparation containing hemocoagulase derived from the snake Bothrops atrox, which is batroxobin—a thrombin-like enzyme with coagulant activity. 1

Active Ingredient Clarification

  • Batroxobin is the active enzyme in Botroclot, isolated from Bothrops atrox (also known as Bothrops atrox moojeni) snake venom 2, 1
  • The term "gem coagulase" does not appear in medical literature as a recognized coagulation enzyme
  • Batroxobin is classified as a snake venom-derived thrombin-like serine protease with distinct hemostatic properties 3

Mechanism of Action

Batroxobin functions differently from thrombin:

  • Cleaves fibrinopeptide A from fibrinogen to convert it into fibrin, forming clots 3, 4
  • Unlike thrombin, batroxobin does not cleave fibrinopeptide B (though some sources indicate it cleaves both A and B peptides, contrasting with ancrod which only cleaves A) 2
  • Maintains coagulant activity even in the presence of heparin, making it useful in anticoagulated patients 3
  • Does not activate factor XIII, resulting in fibrin clots that are more susceptible to fibrinolysis 2

Clinical Applications of Botroclot

The formulation is used for:

  • Dental surgeries and minor surgical wounds as its primary indication 1
  • Emerging evidence suggests potential benefit in diabetic wound healing, where topical application improved wound closure, decreased inflammatory markers, and increased collagen deposition in animal models 1
  • Related batroxobin preparations have been studied in deep vein thrombosis, stroke, and other thrombotic conditions 2, 5

Important Distinction from Standard Anticoagulants

  • Batroxobin is not endorsed by major guidelines (American College of Chest Physicians, American Heart Association) as a standard antithrombotic agent 6
  • Guideline-endorsed thrombolytics include tPA (alteplase), reteplase, tenecteplase, streptokinase, and urokinase with established safety profiles 6, 7
  • Batroxobin's mechanism as a defibrinogenating enzyme differs fundamentally from standard anticoagulants like heparin, which work through antithrombin potentiation 8

References

Research

Defibrinogenating enzymes.

Drugs, 1997

Research

Slounase, a Batroxobin Containing Activated Factor X Effectively Enhances Hemostatic Clot Formation and Reducing Bleeding in Hypocoagulant Conditions in Mice.

Clinical and applied thrombosis/hemostasis : official journal of the International Academy of Clinical and Applied Thrombosis/Hemostasis, 2021

Research

Batroxobin mobilizes circulating endothelial progenitor cells in patients with deep vein thrombosis.

Clinical and applied thrombosis/hemostasis : official journal of the International Academy of Clinical and Applied Thrombosis/Hemostasis, 2011

Guideline

Nattokinase and Antithrombotic Therapy

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Guideline

Alteplase Pharmacokinetics and Clinical Implications

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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