What are the post-vaccination serology results for Hepatitis B (HBV)?

Medical Advisory BoardAll articles are reviewed for accuracy by our Medical Advisory Board
Article Review Status
Submitted
Under Review
Approved

Last updated: April 2, 2025 • View editorial policy

From the Guidelines

Post-vaccination serology for hepatitis B should be performed 1-2 months after completing the three-dose vaccination series to confirm immunity, with a focus on healthcare workers, immunocompromised individuals, sexual partners of hepatitis B carriers, and others at high risk of exposure, as recommended by the most recent guidelines 1. The test measures hepatitis B surface antibody (anti-HBs) levels, with results ≥10 mIU/mL indicating successful immunization and protection against infection.

  • Key groups that require post-vaccination serology include: + Healthcare workers + Immunocompromised individuals + Sexual partners of hepatitis B carriers + Hemodialysis patients + HIV-infected persons
  • If antibody levels are inadequate (<10 mIU/mL), revaccination with another complete three-dose series is recommended, followed by repeat serology testing, as supported by studies 2, 3, 4.
  • The immune response to hepatitis B vaccination varies based on age, weight, smoking status, and immune function, with approximately 5-10% of healthy adults failing to develop adequate antibody levels after standard vaccination, as noted in previous research 5.
  • For those who develop adequate antibody levels, immunity is generally long-lasting, and routine booster doses are not recommended for immunocompetent individuals, according to the latest guidelines 1.

From the Research

Post-Vaccination Serology for Hepatitis B

  • The persistence of immunity provided by hepatitis B (Hep B) vaccine 18-26 years after primary vaccination was evaluated in a cross-sectional study of 628 participants 6.
  • The study found that 55.4% of participants had anti-HBs levels of ≥10 mIU/mL, with a geometric mean concentration (GMC) of 82.59 mIU/mL.
  • Among participants with anti-HBs levels of <10 mIU/mL, 93.1% reached anti-HBs levels of ≥10 mIU/mL after a booster dose, with a GMC of 493.47 mIU/mL 6.
  • The Centers for Disease Control and Prevention (CDC) recommends postvaccination serologic testing (antibody to hepatitis B surface antigen [anti-HBs]) 1-2 months after the final dose of vaccine is administered 7.
  • A longitudinal study demonstrated long-lasting hepatitis B virus (HBV) cellular immunity despite loss of antibody against HBV surface antigen, with all 44 participants testing positive for tumor necrosis factor α, interleukin 10, or interleukin 6 production by HBV surface antigen-specific T cells 8.
  • Seroconversion rates after hepatitis B vaccination were evaluated in patients with end-stage renal disease, with 83% of patients seroconverting after three doses of Heplisav-B, a recombinant adjuvanted vaccine 9.
  • The American College of Physicians and the Centers for Disease Control and Prevention recommend vaccinating against hepatitis B virus (HBV) in all unvaccinated adults at risk for infection, and screening for HBV in high-risk persons 10.

Key Findings

  • Booster doses can increase anti-HBs levels in participants with initial levels of <10 mIU/mL 6.
  • Long-lasting cellular immunity against HBV can persist despite loss of antibody against HBV surface antigen 8.
  • Hepatitis B vaccination is recommended for all unvaccinated adults at risk for infection, and screening for HBV is recommended for high-risk persons 10.
  • Seroconversion rates after hepatitis B vaccination can be high in patients with end-stage renal disease, with 83% of patients seroconverting after three doses of Heplisav-B 9.

References

Guideline

sexually transmitted infections treatment guidelines, 2021.

MMWR Recommendations and Reports, 2021

Research

CDC guidance for evaluating health-care personnel for hepatitis B virus protection and for administering postexposure management.

MMWR. Recommendations and reports : Morbidity and mortality weekly report. Recommendations and reports, 2013

Research

Seroconversion following Heplisav-B, hepatitis B vaccine (recombinant), adjuvanted, in patients with end-stage renal disease at an urban safety net hospital.

American journal of health-system pharmacy : AJHP : official journal of the American Society of Health-System Pharmacists, 2023

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.