What is the role of Tenecteplase (TNK-tissue plasminogen activator) in the management of acute ischemic stroke?

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Last updated: November 25, 2025View editorial policy

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Tenecteplase (TNK) for Acute Ischemic Stroke

Tenecteplase is now a suitable alternative to alteplase for acute ischemic stroke treatment within 4.5 hours of symptom onset, with the advantage of single-bolus administration and superior recanalization rates, particularly in patients with large vessel occlusion. 1, 2

Key Pharmacologic Advantages

Tenecteplase offers significant practical benefits over alteplase:

  • Single-bolus administration (0.25 mg/kg, maximum 25 mg) versus alteplase's 1-hour infusion protocol, reducing nursing time and medication errors 1, 3
  • Longer half-life (90-130 minutes) compared to alteplase, allowing for simplified dosing 1
  • Greater fibrin specificity with reduced systemic coagulopathy 4, 5
  • Superior recanalization rates (22% vs 10% substantial reperfusion) prior to mechanical thrombectomy 1

Weight-Based Dosing Protocol

Administer tenecteplase as a single IV bolus using the following weight-based dosing 1:

  • <60 kg: 30 mg
  • 60-69 kg: 35 mg
  • ≥70 kg: Standard 0.25 mg/kg (maximum 25 mg)

For comparison, alteplase requires 0.9 mg/kg (maximum 90 mg) with 10% as initial bolus over 1 minute, then 90% infused over 60 minutes 3

Clinical Efficacy Evidence

The highest quality recent evidence demonstrates noninferiority to alteplase:

  • ORIGINAL trial (2024): Tenecteplase achieved 72.7% excellent functional outcomes (mRS 0-1) versus 70.3% with alteplase, meeting noninferiority criteria 2
  • Meta-analysis of 5 trials: TNK showed 57.9% disability-free outcomes versus 55.4% with alteplase, with the lower confidence interval well within noninferiority margins 6
  • Recanalization superiority: TNK demonstrates higher recanalization rates without increasing intracerebral hemorrhage risk 5, 7

Guideline Recommendations and Time Windows

Current guidelines support tenecteplase use with specific considerations:

  • Within 3 hours: Both agents are strongly recommended (Level A evidence) 3
  • 3-4.5 hours: Both agents are conditionally recommended for carefully selected patients (Level B evidence) 3
  • AHA/ASA position: Tenecteplase may be considered as an alternative to alteplase, particularly in patients with minor neurological impairment and no major intracranial occlusion (Class IIb, Level B-R) 1

Critical caveat: Even if endovascular therapy is planned, eligible patients should still receive IV thrombolysis without delay 1

Blood Pressure Requirements

Identical strict blood pressure criteria apply to both agents 3:

Pre-treatment (absolute contraindication if not met):

  • SBP must be <185 mmHg
  • DBP must be <110 mmHg

Post-treatment targets for 24 hours:

  • SBP ≤180 mmHg
  • DBP ≤105 mmHg

Monitoring frequency 3:

  • Every 15 minutes for first 2 hours
  • Every 30 minutes for hours 2-8
  • Hourly from hours 8-24

Safety Profile

Both agents share similar safety profiles:

  • Symptomatic ICH rate: 1.2% for both tenecteplase and alteplase in head-to-head comparison 2
  • 90-day mortality: 4.6% with tenecteplase versus 5.8% with alteplase 2
  • Shared contraindications: Prior intracranial hemorrhage, recent significant trauma/surgery, uncontrolled hypertension 1

Practical Clinical Advantages

Tenecteplase offers workflow benefits that make it particularly attractive:

  • Faster administration in centers considering endovascular therapy or patient transfer 1
  • Reduced complexity eliminates infusion pump setup and monitoring 1
  • Lower risk of dosing errors with single-bolus administration 1

Agents to Avoid

Never use streptokinase due to unacceptably high hemorrhage rates 1. Other agents (reteplase, urokinase, anistreplase) lack extensive stroke testing and are not recommended 1.

Post-Thrombolytic Management

Regardless of agent chosen 3:

  • Admit to ICU or stroke unit for minimum 24 hours intensive monitoring
  • Neurological assessments every 15 minutes during/immediately after administration
  • Every 30 minutes for 6 hours post-treatment
  • Hourly from hours 6-24

If symptomatic ICH suspected: Immediately discontinue infusion, obtain emergent CT, consider cryoprecipitate for fibrinogen replacement, and obtain neurosurgical consultation 3

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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