What are the guideline-directed pre-pregnancy laboratory (lab) screenings?

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Last updated: February 12, 2025View editorial policy

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From the Guidelines

Guideline-directed pre-pregnancy laboratory screenings are primarily focused on screening for gestational diabetes and genetic carrier status.

Key Screenings

  • Gestational diabetes screening: Fasting glucose should be measured at the first prenatal visit (no later than 20 weeks’ gestation) 1. A 75-g oral glucose tolerance test (OGTT) should be performed if the fasting glucose concentration is greater than 85 mg per dL (4.7 mmol per L) 1.
  • Genetic carrier screening: Carrier testing of prospective parents, including biologic fathers, should be undertaken in the safest and most cost-effective way 1. This is particularly important for genetic conditions that may require testing of parents and other family members to determine reproductive risks.

Patient Selection

  • Low-risk women: May be excluded from glucose testing if they meet certain criteria, such as age younger than 25 years, BMI of 25 kg per m2 or less, no history of abnormal OGTT result, and no first-degree relative with diabetes 1.
  • High-risk women: Should undergo glucose testing as soon as possible if they have clinical characteristics consistent with a high risk of gestational diabetes, such as marked obesity or a personal history of gestational diabetes 1.

Testing Timing

  • First prenatal visit: Fasting glucose should be measured, and genetic carrier screening should be considered 1, 1.
  • 24 to 28 weeks’ gestation: Glucola test or OGTT should be performed for women who have not previously been screened for gestational diabetes 1.

From the Research

Guideline-Directed Pre-Pregnancy Laboratory Screenings

The following laboratory screenings are recommended for pregnant women:

  • Blood glucose testing to detect gestational diabetes mellitus 2
  • Testing for hepatitis B, Chlamydia trachomatis infection, or human immunodeficiency virus infections for women at risk 2
  • Alpha fetoprotein testing for detection of neural tube defects 2
  • Aneuploidy screening or diagnostic testing, which may include traditional serum analyte screening, cell-free DNA, chorionic villus sampling, or amniocentesis 3
  • Carrier screening, which may include testing for genetic disorders such as cystic fibrosis or sickle cell disease 4

Screening Methods and Interpretation

Methods of screenings involve measuring antigen or antibody titers, a combination of antigen/antibody titers, or by specialized genetic tests 5. Providers responsible for pregnant women should be able to identify which diseases they need to screen for and how to interpret the findings 5. Neonatal providers should also be able to interpret the findings and manage neonates appropriately 5.

Recommendations and Guidelines

The American Congress of Obstetricians and Gynecologists recommends that all pregnant women be offered aneuploidy screening or diagnostic testing 3. Recent national society guidelines on prepregnancy genetic screening and prenatal diagnosis for aneuploidy have been published, and obstetric care providers should educate themselves about the most up-to-date recommendations 4. Society guidelines are largely in agreement, but some newer technologies are not yet routinely recommended for widespread use due to lack of clinical trials and validation studies 4.

References

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Research

Prenatal Diagnosis: Screening and Diagnostic Tools.

Obstetrics and gynecology clinics of North America, 2017

Research

Maternal Prenatal Screening and Serologies.

Advances in neonatal care : official journal of the National Association of Neonatal Nurses, 2018

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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