What is the half-life of tenecteplase (tissue plasminogen activator, tPA)?

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Tenecteplase Half-Life

Tenecteplase has a terminal phase half-life of 90 to 130 minutes after intravenous bolus administration, which is substantially longer than alteplase and allows for convenient single-bolus dosing. 1, 2

Pharmacokinetic Profile

The half-life of tenecteplase is well-established across multiple authoritative sources:

  • The FDA drug label confirms a terminal phase half-life of 90 to 130 minutes following IV bolus administration, with linear pharmacokinetics across the 30,40, and 50 mg dose range 2
  • The American Heart Association recognizes this 90-130 minute half-life as the key pharmacokinetic feature that distinguishes tenecteplase from alteplase 1
  • This extended half-life results from genetic modifications to the native tissue plasminogen activator molecule, including amino acid substitutions that provide approximately 14-fold greater fibrin specificity and slower plasma clearance 3

Clinical Significance of the Extended Half-Life

The longer half-life translates directly into practical clinical advantages:

  • Single-bolus administration is possible (typically given over 5 seconds), eliminating the need for the 1-hour infusion required with alteplase 1, 4
  • Workflow advantages are significant, particularly in centers considering endovascular therapy or patient transfer, as nursing time is reduced and potential medication errors are minimized 4
  • The plasma clearance ranges from 99 to 119 mL/min, with liver metabolism serving as the major clearance mechanism 2

Comparison to Alteplase

The half-life difference between these agents is clinically meaningful:

  • Alteplase requires continuous infusion over 60 minutes due to its shorter half-life (approximately 4-5 minutes for the initial phase) 4
  • Tenecteplase's 90-130 minute half-life is approximately 18 minutes in some references, though the terminal elimination phase extends to the 90-130 minute range 3, 2
  • Despite the longer half-life, tenecteplase maintains similar safety profiles to alteplase, with equivalent rates of symptomatic intracranial hemorrhage (1.2% in both groups) 5

References

Guideline

Tenecteplase Pharmacokinetics and Clinical Significance

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Research

Tenecteplase: a review.

Clinical therapeutics, 2001

Guideline

Tenecteplase vs Alteplase for Acute Ischemic Stroke Management

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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