How often should patients undergo syphilis and HIV screening?

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Last updated: December 3, 2025View editorial policy

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HIV and Syphilis Screening Frequency

Screen for HIV and syphilis at least annually if you are sexually active, with more frequent screening every 3-6 months if you have any high-risk behaviors.

Standard Annual Screening for All Sexually Active Individuals

  • All sexually active adults should receive HIV screening at least once annually, regardless of perceived risk, as recommended by the CDC 1.
  • All sexually active men who have sex with men (MSM) require annual syphilis serologic testing at minimum 1.
  • For HIV-infected individuals who are sexually active, annual screening for both syphilis and other STIs is mandatory 1, 2.

Increased Frequency Screening Every 3-6 Months

You need more frequent screening (every 3-6 months) if you have ANY of these risk factors:

  • Multiple or anonymous sexual partners 1, 3
  • Unprotected intercourse outside a mutually monogamous relationship 1
  • Sex in conjunction with illicit drug use (particularly methamphetamine) 1, 3
  • Partners who engage in any of these high-risk activities 1
  • Recent diagnosis of any STI 3, 4
  • Sex work or exchanging sex for money or drugs 1, 3
  • Previous syphilis infection 5

The evidence strongly supports this increased frequency: Studies in HIV-positive men and MSM demonstrate that screening every 3 months versus annually detects significantly more early syphilis cases (53% vs 16% asymptomatic detection, P=0.001) 5. This more frequent interval catches infections earlier when they are more treatable and less likely to cause complications.

Special Considerations for HIV-Infected Individuals

  • HIV-infected persons require at minimum annual syphilis screening, but the threshold should be lower for moving to 3-6 month intervals given the bidirectional relationship between HIV and syphilis 1.
  • The reinfection rate for syphilis is extremely high at 42.7% in high-risk populations, making frequent rescreening critical 6.
  • Testing should include both treponemal and nontreponemal tests (such as RPR/VDRL plus EIA/CIA) for accurate diagnosis 1, 3.

Practical Algorithm for Your Situation

If you are sexually active with only one mutually monogamous partner and no other risk factors:

  • Screen annually for both HIV and syphilis 1

If you have even ONE of the high-risk factors listed above:

  • Screen every 3-6 months for both HIV and syphilis 1, 3, 5

If you are HIV-positive:

  • Screen at least annually, but strongly consider every 3-6 months given your increased vulnerability 1

Critical Pitfalls to Avoid

  • Don't assume you're low-risk based on sexual orientation identity alone—your actual sexual practices and partner behaviors determine your screening needs 3.
  • Don't skip screening just because you lack symptoms—the majority of syphilis cases detected through screening are asymptomatic, and nearly half (47%) of new diagnoses occur during routine screening rather than symptomatic presentation 7.
  • Don't rely on a single positive test—syphilis diagnosis requires both treponemal AND nontreponemal testing for confirmation 1.
  • Don't forget that the window period matters for HIV—if you had a recent high-risk exposure, you need follow-up testing at 3 months even if initial testing is negative 8.

When to Rescreen After Last Test

Since you mentioned your last screening was in [YEAR], if that was more than 12 months ago, you need screening now. If you have any of the high-risk factors listed above and it's been more than 3-6 months, you should be screened immediately 1, 3, 5.

References

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Guideline

STI Screening Recommendations for Bisexual Individuals

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Guideline

STI Screening Recommendations

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Research

[Syphilis Testing and High Rate of Infection Among HIV-infected Patients].

Kansenshogaku zasshi. The Journal of the Japanese Association for Infectious Diseases, 2016

Guideline

Screening for Sexually Transmitted Infections after a Risky Sexual Encounter

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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