What are the diagnostic investigations for Addison's disease (Adrenal Insufficiency)?

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From the Guidelines

Investigations for Addison's disease should include both screening tests and confirmatory tests to establish the diagnosis, with the short Synacthen (ACTH) stimulation test being the definitive diagnostic test, as recommended by the most recent guidelines 1.

Initial Screening

Initial screening should involve measuring morning serum cortisol and plasma ACTH levels, with low cortisol (<140 nmol/L) and elevated ACTH (>80 ng/L) suggesting primary adrenal insufficiency, as noted in the consensus statement on the diagnosis, treatment, and follow-up of patients with primary adrenal insufficiency 1.

  • Electrolyte abnormalities including hyponatremia, hyperkalemia, and metabolic acidosis may be present.
  • The high-dose (250-μg) ACTH stimulation test is the most popular diagnostic test for adrenal insufficiency, and its use is suggested due to easier practical modalities and comparable accuracy to the low-dose test 1.

Confirmatory Tests

The short Synacthen (ACTH) stimulation test is the definitive diagnostic test, where 250 μg of synthetic ACTH is administered intravenously or intramuscularly, with cortisol levels measured at baseline, 30 minutes, and 60 minutes, as described in the guidelines for the diagnosis and management of critical illness-related corticosteroid insufficiency (CIRCI) 1.

  • A peak cortisol response below 500-550 nmol/L confirms adrenal insufficiency.
  • Once adrenal insufficiency is confirmed, determining the etiology is important, with adrenal antibodies being checked to identify autoimmune adrenalitis, the most common cause, as outlined in the classification and causes of primary adrenal insufficiency 1.

Additional Tests

Additional tests may include:

  • Imaging studies, including abdominal CT scan, to detect adrenal calcification, hemorrhage, or infiltrative processes, as recommended in the consensus statement on the diagnosis, treatment, and follow-up of patients with primary adrenal insufficiency 1.
  • Very long-chain fatty acid levels should be measured to rule out adrenoleukodystrophy in younger patients, as suggested in the guidelines for the diagnosis and management of CIRCI 1.
  • Tuberculosis screening, fungal serologies, and HIV testing may be necessary depending on clinical suspicion, as noted in the classification and causes of primary adrenal insufficiency 1. These investigations are crucial as early diagnosis allows for appropriate hormone replacement therapy with hydrocortisone and fludrocortisone to prevent life-threatening adrenal crisis, as emphasized in the consensus statement on the diagnosis, treatment, and follow-up of patients with primary adrenal insufficiency 1.

From the Research

Investigations for Addison's Disease

To investigate Addison's disease, the following steps can be taken:

  • A high clinical suspicion is needed to avoid misdiagnosing a life-threatening adrenal crisis 2
  • Clinical manifestations before an adrenal crisis can include hyperpigmentation, fatigue, anorexia, orthostasis, nausea, muscle and joint pain, and salt craving 2
  • Cortisol levels decrease and adrenocorticotropic hormone levels increase 2
  • A cosyntropin stimulation test can be used to confirm the diagnosis when clinically suspected 2

Diagnostic Strategies

Diagnostic strategies for Addison's disease may include:

  • Standard care treatments, including the management of patients during pregnancy and adrenal crises 3
  • Emerging therapies designed to improve the quality of life and new strategies to modify the natural history of autoimmune AD 3
  • Genetic diagnosis, especially in pediatric cases 4
  • Awareness of unusual presentations and the importance of pediatric and adult awareness 4

Management and Treatment

Management and treatment of Addison's disease may involve:

  • Replacement of mineralocorticoids and glucocorticoids 2
  • Stress-dose glucocorticoids during times of stress, such as illness or invasive surgical procedures 2
  • Continuous education of patients and health care professionals on the ever-present danger of adrenal crisis 3
  • Research on immune checkpoint inhibitors, which can cause a large panel of endocrine side effects, including Addison's disease 4

References

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Research

Epidemiology, pathogenesis, and diagnosis of Addison's disease in adults.

Journal of endocrinological investigation, 2019

Research

Addison's Disease: Diagnosis and Management Strategies.

International journal of general medicine, 2023

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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