What is the recommended dose of tPA (tissue plasminogen activator) for acute ischemic stroke?

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Last updated: December 6, 2025View editorial policy

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Stroke tPA Dose

The recommended dose of tPA (alteplase) for acute ischemic stroke is 0.9 mg/kg with a maximum total dose of 90 mg, administered as 10% IV bolus over 1 minute followed by 90% infusion over 60 minutes. 1, 2

Standard Dosing Protocol

  • Total dose: 0.9 mg/kg (maximum 90 mg) 1, 2
  • Initial bolus: 10% of total calculated dose given IV over 1 minute 2, 3
  • Remaining infusion: 90% of total dose infused over 60 minutes 2, 3

This dosing regimen is based on the landmark NINDS trial that demonstrated improved functional outcomes at 3 months when tPA was administered within 3 hours of symptom onset. 1

Time Window Considerations

The dosing remains the same regardless of time window, but eligibility criteria differ:

  • 0-3 hours: Level A recommendation - Offer tPA to all patients meeting NINDS inclusion/exclusion criteria (absolute benefit: 12% more patients achieve minimal/no disability, NNT=8) 1, 3
  • 3-4.5 hours: Level B recommendation - Consider tPA for carefully selected patients meeting ECASS III criteria (smaller benefit with NNT=14 for favorable outcome) 1, 3
  • Beyond 4.5 hours: Contraindicated - Do not administer IV tPA (Grade 1B recommendation against use) 3, 4

Critical Dosing Considerations

Maximum dose cap is essential: The 90 mg maximum dose limit must be strictly observed. Patients weighing >100 kg receive a lower per-kilogram dose (0.82 mg/kg vs 0.90 mg/kg), but research supports maintaining this cap as patients >100 kg who received lower per-kilogram doses had higher rates of symptomatic ICH (2.6% vs 1.7%) and mortality despite similar functional outcomes. 5

Do not modify the dose: Lower doses (such as 0.6 mg/kg) studied in patients beyond 3 hours showed an 11% symptomatic hemorrhage rate with only 14% early major improvement, demonstrating that dose reduction does not improve the safety profile. 6

Safety Profile with Standard Dosing

  • Baseline symptomatic ICH rate: 4-6% with proper dosing 2, 3
  • NINDS trial data: 6.4% symptomatic ICH in tPA-treated patients versus 0.6% in placebo 2
  • Patients on antiplatelet therapy: 3% absolute increased risk of symptomatic ICH but can still receive standard dosing 2, 3

Absolute Contraindications to Standard Dosing

Never administer tPA to patients on direct oral anticoagulants (DOACs) like apixaban due to substantially elevated bleeding risk. 2, 3

Administration Timing

Once the decision is made to administer tPA, treat as rapidly as possible within the appropriate time window, as earlier treatment results in greater proportional benefit. 1, 2, 3

References

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Guideline

Loading Dose of tPA for Acute Ischemic Stroke

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Guideline

Acute Ischemic Stroke Treatment Guidelines

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Guideline

Thrombolysis After 4.5 Hours for Acute Ischemic Stroke

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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