Can herpes simplex virus (HSV) cause a positive Antinuclear Antibody (ANA) test?

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From the Guidelines

Herpes infections are unlikely to directly cause a positive ANA test, as ANA tests are primarily associated with autoimmune disorders rather than viral infections. The ANA test detects antibodies that target components within the cell nucleus, and its positivity is more commonly linked to conditions like lupus, rheumatoid arthritis, or Sjögren's syndrome 1. However, it's possible for viral infections, including herpes, to trigger a temporary autoimmune-like response, potentially leading to a transiently positive ANA test. This occurs because viral infections can broadly stimulate the immune system, sometimes causing it to produce autoantibodies as a secondary effect.

Some key points to consider:

  • The specificity of ANA detection for systemic lupus erythematosus (SLE) is relatively low, at 74.7% when using a cut-off point of 1:80 by the indirect immunofluorescence test on HEp-2 cells (IFI) 1.
  • ANA tests are requested in various clinical scenarios, including diagnosis, ruling out autoimmune disorders, and treatment evaluation unrelated to SLE.
  • The high sensitivity and relatively low specificity of ANA tests make them suitable as an entry criterion for the EULAR/ACR 2019 classification of SLE, but their use in diagnosis or screening should be approached with caution.

If you have herpes and receive a positive ANA test, it's crucial to consult with a healthcare provider for proper interpretation, as the positive result is more likely related to another underlying condition rather than the herpes infection itself. Your doctor may recommend additional testing to determine the cause of the positive ANA, especially if you have symptoms suggesting an autoimmune disorder. The positive ANA result should be evaluated in the context of your complete clinical picture, including symptoms, medical history, and other laboratory findings.

From the Research

Herpes and ANA Test Results

  • Herpes can potentially cause a positive ANA test result, as certain infections can trigger the production of antinuclear antibodies 2.
  • A positive ANA test may be seen with non-autoimmune inflammatory diseases, including both acute and chronic infections, such as herpes 2, 3.
  • The ANA test can be a useful laboratory tool to help confirm or exclude the diagnosis of systemic rheumatic disease, but a positive result can be difficult to interpret due to the relatively high prevalence of ANAs in other inflammatory conditions and healthy individuals 3.

Mechanism of Herpes Infection and ANA Test Results

  • Herpes simplex virus (HSV) infection can stimulate the production of IgG antibodies, which can be detected by the ANA test 4.
  • Long-term acyclovir chemosuppression can reduce humoral immunity to HSV, but antibody concentrations can increase following the first untreated recurrence 4.

Interpretation of ANA Test Results

  • Understanding and interpreting ANA test results requires insight into the sensitivity and specificity of each assay, as well as the importance of the disease context and antibody level 5.
  • The clinical relevance of the differences between techniques for detecting antibodies to clinically relevant antigens should be considered when interpreting ANA test results 5.

References

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Research

ANA testing in the presence of acute and chronic infections.

Journal of immunoassay & immunochemistry, 2016

Research

Antinuclear antibody test.

Australian family physician, 2013

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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