What is the recommended loading dose of tissue plasminogen activator (tPA) for acute ischemic stroke?

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Last updated: December 10, 2025View editorial policy

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Loading Dose of tPA for Acute Ischemic Stroke

The recommended loading dose of tissue plasminogen activator (tPA) for acute ischemic stroke is 0.9 mg/kg (maximum 90 mg total), with 10% of the calculated dose given as an intravenous bolus over 1 minute, followed by the remaining 90% infused over 60 minutes. 1

Standard Dosing Protocol

  • Total dose: 0.9 mg/kg body weight, not to exceed 90 mg maximum 1, 2
  • Initial bolus: 10% of total calculated dose administered intravenously over 1 minute 1, 3
  • Continuous infusion: Remaining 90% infused over 60 minutes 1, 3

For example, a 70 kg patient would receive:

  • Total dose: 63 mg (70 kg × 0.9 mg/kg)
  • Bolus: 6.3 mg over 1 minute
  • Infusion: 56.7 mg over 60 minutes

Time Window for Administration

The strength of recommendation varies significantly based on time from symptom onset:

  • 0-3 hours: Strong recommendation (Grade 1A) - This is the FDA-approved window with the strongest evidence for benefit 1, 4
  • 3-4.5 hours: Conditional recommendation (Grade 2C) - Treatment is reasonable but requires more careful patient selection 1, 4
  • Beyond 4.5 hours: Strong recommendation AGAINST treatment (Grade 1B) - Do not administer tPA beyond this window 1, 4

The ECASS III trial extended the treatment window to 4.5 hours, but used more restrictive patient selection criteria than current U.S. practice for the 0-3 hour window. 5

Evidence Supporting Standard Dose

Do not use lower doses of tPA. A large Chinese registry study (TIMS-China) with 919 patients demonstrated that the standard 0.85-0.95 mg/kg dose resulted in significantly better functional outcomes compared to lower doses:

  • Patients receiving 0.5-0.7 mg/kg had 41.89% excellent recovery (mRS 0-1) versus 53.83% with standard dose (OR 0.58, p=0.031) 6
  • Patients receiving 0.7-0.85 mg/kg had 54.33% functional independence (mRS 0-2) versus 64.51% with standard dose (OR 0.66, p=0.036) 6
  • No significant difference in symptomatic intracranial hemorrhage or mortality between dose groups 6

This finding is particularly important because some Asian populations were previously thought to potentially benefit from lower doses, but the evidence clearly supports 0.9 mg/kg as optimal. 6, 7

Critical Safety Considerations

Symptomatic intracranial hemorrhage (sICH) rates:

  • Baseline rate with proper dosing: 4-6% 1
  • NINDS trial: 6.4% with tPA versus 0.6% with placebo 1
  • Real-world practice: 7% in early clinical implementation 2

Patients on antiplatelet therapy prior to stroke have a 3% absolute increased risk of symptomatic ICH but should still receive tPA if otherwise eligible. 1

Patients on direct oral anticoagulants (DOACs) should NOT receive tPA as routine practice due to substantially elevated bleeding risk. 8, 1

Pre-Treatment Requirements

Before administering the loading dose:

  • Blood pressure must be controllable to <185/110 mmHg 3
  • Insert all necessary IV lines, Foley catheter, and indwelling tubes BEFORE tPA administration to minimize trauma and bleeding risk 3
  • Complete dysphagia screening before any oral medications 8
  • Exclude intracranial hemorrhage on non-contrast head CT 3

Post-Loading Dose Management

Monitoring intensity after bolus administration:

  • Every 15 minutes during the 60-minute infusion 3
  • Every 15 minutes for 2 hours after infusion completion 3
  • Every 30 minutes for the next 6 hours 3
  • Hourly for 16 hours thereafter 3

Obtain urgent head CT if patient develops:

  • Acute neurological deterioration 3
  • Severe headache 3
  • Acute hypertension 3
  • Nausea or vomiting 3

Delay aspirin until 24 hours after tPA administration and after repeat imaging excludes intracranial hemorrhage. 8, 1

Common Pitfalls to Avoid

Do not exceed 90 mg total dose even in patients weighing >100 kg, as higher doses increase hemorrhage risk without improving outcomes. 1

Do not use lower doses in Asian patients - the evidence clearly demonstrates that 0.9 mg/kg is superior to lower doses even in Chinese populations. 6, 7

Do not delay tPA administration to obtain vascular imaging if the patient meets clinical criteria for thrombolysis within the time window. 3

Do not give tPA if blood pressure cannot be reduced below 185/110 mmHg despite antihypertensive therapy. 3

References

Guideline

Loading Dose of tPA for Acute Ischemic Stroke

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Guideline

Management of Acute Ischemic Stroke in Newly Diagnosed Polycythemia Vera

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Guideline

Thrombolysis After 4.5 Hours for Acute Ischemic Stroke

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Research

Efficacy and safety of different doses of intravenous tissue plasminogen activator in Chinese patients with ischemic stroke.

Journal of clinical neuroscience : official journal of the Neurosurgical Society of Australasia, 2010

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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