How to Use Regenin Fill (Hyaluronic Acid) for Dermal Filling

Inject intradermally using a 30-gauge needle at shallow angles, creating visible wheals to confirm proper depth and avoid vascular complications. 1

Anatomical Knowledge is Non-Negotiable

Master the vascular anatomy of the injection site before proceeding—vessels can be encountered within 1mm of your injection point 2
Identify the location of major vessels (facial artery, transverse facial artery) which reside in the hypodermis, well below your target injection plane 1
For lip injections specifically, maintain depth above the orbicularis oris muscle to avoid vascular structures 2

Patient Discussion Points

Explain the procedure details, expected duration of effect, and potential risks before treatment 3
Warn about expected minor complications: bruising (7-9 days) and edema that resolve spontaneously 2

Needle Selection and Depth

Use 30-gauge, 4mm length needles for intradermal placement 1
Insert at shallow angles with bevel facing upward 1
Target the dermis layer only—stay superficial to avoid the hypodermis where major vessels reside 1

Critical Safety Protocol During Injection

Aspirate before each injection to detect intravascular placement and avoid introducing filler directly into vessels 1
Create visible papules or wheals (6-10 mm diameter) with blanching during injection—this confirms proper intradermal placement and proves you are NOT in the hypodermis 1
Use incremental injections rather than large boluses to minimize vascular compression and allow early detection of complications 1

Volume and Distribution

For lip injections: divide into quadrants (upper lip: 2 quadrants, lower lip: 2 quadrants) 2
Inject 0.2-0.5 mL per quadrant to avoid compression of neurovascular structures 2
Use retrograde injection technique when using needle approach 2

For Lip Augmentation

Enhance, do not distort, the vermilion border (white roll)—even minor misalignment creates visible asymmetry 2
Preserve philtral columns as natural boundaries 2
Maintain the sublabial concavity to avoid unnatural "duck lip" appearance 2

Depth Errors

Never inject deep enough to reach the hypodermis—this is where the transverse facial artery and other major vessels reside 1
Failure to create visible wheals indicates you may be injecting too deep 1

Technical Errors

Avoid large bolus injections that can compress vessels 1
Do not skip aspiration—this is your primary defense against intravascular injection 1

Hyaluronic Acid Properties

HA is naturally occurring in skin with negligible risk of immunologic or allergic reactions 4
Duration of effect may exceed expected bioavailability due to collagen synthesis and fibroblast stimulation 4
Rapidly reversible with hyaluronidase injection if complications occur 4
Works by filling, volumizing, and hydrating tissue through water affinity 4