What is the recommended treatment regimen for H pylori (Helicobacter pylori) infection?

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Last updated: December 10, 2025View editorial policy

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H. pylori Treatment Regimen

Bismuth quadruple therapy for 14 days is the recommended first-line treatment for H. pylori infection, consisting of a PPI twice daily, bismuth 300mg four times daily, metronidazole 500mg three to four times daily, and tetracycline 500mg four times daily. 1

First-Line Treatment: Bismuth Quadruple Therapy

The American Gastroenterological Association recommends this regimen because it achieves 80-90% eradication rates even in areas with high clarithromycin and metronidazole resistance. 1, 2 This is critical because clarithromycin resistance now exceeds 15% in most regions of North America, making traditional triple therapy unacceptably ineffective. 2

Specific dosing components:

  • PPI: Standard dose twice daily (pantoprazole 40mg, lansoprazole 30mg, omeprazole 20mg, esomeprazole 20mg, dexlansoprazole 30mg, or rabeprazole 20mg) 1
  • Bismuth: Bismuth subsalicylate 262mg or bismuth subcitrate 120mg, four times daily 1
  • Metronidazole: 500mg three to four times daily (total 1.5-2g daily) 1
  • Tetracycline: 500mg four times daily 1

Critical Optimization Factors

PPI administration is crucial: Take twice daily, 30 minutes before meals on an empty stomach, without concomitant antacids. 1 High-dose PPI (esomeprazole or rabeprazole 40mg twice daily) may increase cure rates by an additional 8-12% compared to standard PPIs. 2, 3

14-day duration is mandatory: This improves eradication rates by approximately 5% compared to 7-10 day regimens and is superior across all studies. 1, 2

Why bismuth quadruple therapy works: Bacterial resistance to bismuth is extremely rare, and bismuth's synergistic effect overcomes metronidazole resistance even when present in vitro. 2, 3

Alternative First-Line Option (When Bismuth Unavailable)

Concomitant non-bismuth quadruple therapy for 14 days: 2, 3

  • PPI twice daily
  • Amoxicillin 1000mg twice daily
  • Clarithromycin 500mg twice daily
  • Metronidazole 500mg twice daily

Use this only if: Local clarithromycin resistance is documented <15% and the patient has no prior macrolide exposure for any indication. 2, 3

Second-Line Treatment After First-Line Failure

If bismuth quadruple therapy fails, use levofloxacin triple therapy for 14 days: 1, 3

  • PPI twice daily
  • Amoxicillin 1000mg twice daily 4
  • Levofloxacin 500mg once daily or 250mg twice daily

If clarithromycin-based therapy fails, use bismuth quadruple therapy (if not previously used). 1, 2

Critical antibiotic reuse rules: Never reuse clarithromycin or levofloxacin due to high resistance rates after exposure. Amoxicillin and tetracycline can be reused because resistance remains rare (<5%). 1, 3

Third-Line and Rescue Therapies

After two failed attempts, obtain antibiotic susceptibility testing before further treatment. 1, 2 Molecular testing for clarithromycin and levofloxacin resistance can guide therapy selection earlier. 1

Rifabutin triple therapy for 14 days (third-line option): 1, 3

  • PPI twice daily
  • Amoxicillin 1000mg twice daily
  • Rifabutin 150mg twice daily or 300mg once daily

High-dose dual therapy (rescue option): 1, 3

  • Amoxicillin 2-3 grams daily in 3-4 divided doses
  • PPI high-dose twice daily
  • Duration: 14 days

Special Populations

Penicillin allergy: Bismuth quadruple therapy is the first choice because it contains tetracycline instead of amoxicillin. 1, 3 Consider penicillin allergy testing to enable amoxicillin use, as true allergy is often overreported. 3

Pediatric patients: Treatment should only be conducted by pediatric gastroenterologists in specialist centers. 1

Verification of Eradication

Confirm eradication with urea breath test or monoclonal stool antigen test at least 4 weeks after completing therapy and at least 2 weeks after stopping PPIs. 1, 2 Never use serology for confirmation—antibodies persist long after successful treatment. 3

Common Pitfalls to Avoid

Never assume low clarithromycin resistance without local surveillance data—most regions now exceed 15-20% resistance. 2, 3

Avoid standard once-daily PPI dosing—this is inadequate and reduces eradication rates by 6-10%. 2, 3

Do not use levofloxacin empirically as first-line therapy due to rapidly rising fluoroquinolone resistance rates (11-30% primary, 19-30% secondary). 2

Address compliance issues: More than 10% of patients are poor compliers, which dramatically reduces eradication rates. 2 Diarrhea occurs in 21-41% of patients during the first week; consider adjunctive probiotics to reduce side effects and improve compliance. 2

Patient factors affecting success: Smoking increases failure risk (OR 1.95), and high BMI reduces drug concentrations at the gastric mucosal level. 2

References

Guideline

H. pylori Infection Management

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Guideline

Helicobacter Pylori Infection Treatment Guidelines

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Guideline

H. pylori Treatment Guidelines

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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