What could cause an increase in RBC (Red Blood Cell), hemoglobin (Hb), and hematocrit (Hct) levels over 6 months?

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Evaluation of Rising RBC/Hemoglobin/Hematocrit Over 6 Months

Direct Answer

This patient's rising RBC count (5.96→6.68 million/μL), hemoglobin (17.0→19.0 g/dL), and hematocrit (51.3→57.3%) over 6 months represents clinically significant erythrocytosis that requires immediate investigation for secondary causes—particularly testosterone use, hypoxic conditions, or early polycythemia vera—as these values now exceed normal male ranges and approach thresholds associated with increased thrombotic risk. 1, 2

Clinical Significance of These Values

  • The initial values (Hb 17.0 g/dL, Hct 51.3%) were already at the upper limit of normal for adult males (normal: Hb 15.5±2.0 g/dL, Hct 47±6%), but the progression to Hb 19.0 g/dL and Hct 57.3% now clearly exceeds normal ranges and meets criteria for erythrocytosis (Hb >18.5 g/dL in men, Hct >55%). 1, 2

  • These values are approaching the threshold (Hct >65%, Hb >20 g/dL) where hyperviscosity symptoms and thrombotic complications become significantly more likely, though therapeutic phlebotomy is not yet indicated at this level. 1, 2

Most Likely Causes to Investigate

Testosterone or Androgen Therapy

  • Testosterone replacement therapy is the most common iatrogenic cause of progressive erythrocytosis in this demographic, with erythrocytosis rates up to 66.7% in men on testosterone therapy. 3

  • Intramuscular testosterone formulations carry higher risk (43.8% erythrocytosis rate) compared to transdermal preparations (15.4% rate), with most hemoglobin increases occurring within the first 3 months of therapy. 1

  • Up to 2.7% of men on testosterone develop thromboembolic events, making this a critical diagnosis to confirm or exclude. 3

  • The mechanism involves testosterone's direct stimulation of erythropoiesis, with erythrocytosis being dose-dependent and more common in older patients. 1

Hypoxic Conditions

  • Chronic obstructive pulmonary disease (COPD) causes compensatory erythrocytosis through chronic tissue hypoxia stimulating erythropoietin production. 2

  • Obstructive sleep apnea produces nocturnal hypoxemia that drives sustained erythropoietin production and can cause progressive erythrocytosis over months. 2

  • Smoking causes "smoker's polycythemia" through chronic carbon monoxide exposure, which creates functional hypoxia and stimulates erythropoietin production. 2

Polycythemia Vera

  • JAK2 mutation testing is essential, as up to 97% of polycythemia vera cases carry this mutation, and the progressive nature of this patient's erythrocytosis over 6 months is consistent with a myeloproliferative disorder. 2

  • Polycythemia vera diagnosis requires elevated Hb/Hct plus JAK2 mutation, or elevated Hb/Hct plus two minor criteria (bone marrow hypercellularity, low serum erythropoietin, endogenous erythroid colony formation). 2

Essential Diagnostic Workup

Immediate Laboratory Testing

  • Complete blood count with red cell indices and reticulocyte count to assess bone marrow response and rule out concurrent iron deficiency (which can mask the degree of erythrocytosis). 2

  • Serum ferritin and transferrin saturation are mandatory, as iron deficiency can coexist with erythrocytosis and cause microcytic polycythemia with elevated RBC count but paradoxically lower hemoglobin. 2

  • JAK2 mutation testing (both exon 14 V617F and exon 12) should be ordered immediately to evaluate for polycythemia vera. 2

  • Serum erythropoietin level helps differentiate primary (low EPO in polycythemia vera) from secondary causes (elevated EPO in hypoxic conditions). 2

Clinical History Elements

  • Detailed medication history focusing specifically on testosterone, anabolic steroids, SGLT-2 inhibitors (which cause erythrocytosis in 2.1-22% of users), or erythropoiesis-stimulating agents. 3

  • Smoking history and quantification of pack-years, as carbon monoxide exposure directly stimulates erythropoietin production. 2

  • Symptoms of sleep apnea (witnessed apneas, daytime somnolence, morning headaches) or COPD (chronic dyspnea, productive cough). 2

  • Symptoms suggesting polycythemia vera: aquagenic pruritus (itching after warm shower), erythromelalgia (burning pain in extremities), early satiety or left upper quadrant fullness (splenomegaly). 2

Management Approach Based on Findings

If Testosterone-Related

  • Dose reduction or temporary discontinuation of testosterone is the primary intervention, with close monitoring of hematocrit levels. 1

  • Consider switching from intramuscular to transdermal formulations if testosterone therapy must continue, as this reduces erythrocytosis risk. 1

  • Monitor hematocrit every 3 months initially, then every 6 months once stable, with intervention if Hct exceeds 54%. 1

If Secondary to Hypoxia

  • Treatment of the underlying condition (CPAP for sleep apnea, bronchodilators and smoking cessation for COPD) is the definitive management. 2

  • Smoking cessation alone can resolve "smoker's polycythemia" without additional interventions. 2

If Polycythemia Vera Confirmed

  • Immediate hematology referral is mandatory for patients with positive JAK2 mutation or Hb >20 g/dL with symptoms. 2

  • Therapeutic phlebotomy to maintain hematocrit strictly below 45% is the cornerstone of therapy to reduce thrombotic risk. 2

  • Low-dose aspirin (typically 81 mg daily) is recommended as the second cornerstone of therapy for thrombosis prevention. 2

Critical Safety Thresholds

  • At current levels (Hct 57.3%), this patient has significantly increased blood viscosity and thrombotic risk, particularly if older age, prior VTE history, malignancy, or prolonged immobilization are present. 1

  • Therapeutic phlebotomy becomes indicated only when Hct exceeds 65% with symptoms of hyperviscosity (headache, dizziness, visual disturbances, chest pain), after excluding dehydration. 2

  • If phlebotomy is performed, equal volume replacement with saline or dextrose is mandatory to prevent further hemoconcentration. 2

Common Pitfalls to Avoid

  • Do not perform aggressive phlebotomy at current levels (Hct 57.3%) without first identifying and addressing the underlying cause, as this can induce iron deficiency and worsen oxygen-carrying capacity. 2

  • Avoid assuming this is "normal variation" or "dehydration"—the progressive rise over 6 months with RBC count elevation indicates true erythrocytosis requiring investigation. 2

  • Do not overlook coexisting iron deficiency, which can mask the severity of erythrocytosis by keeping hemoglobin artificially lower while RBC count remains elevated. 2

  • Hemoglobin is more reliable than hematocrit for monitoring, as hematocrit can falsely increase by 2-4% with prolonged sample storage or in hyperglycemia, while hemoglobin remains stable. 1, 2

References

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Guideline

Assessment Protocol for Incidental Erythrocytosis

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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