What is 250 ml of 0.9% sodium chloride (NaCl) solution?

Medical Advisory BoardAll articles are reviewed for accuracy by our Medical Advisory Board
Educational purpose only • Exercise caution as content is pending human review
Article Review Status
Submitted
Under Review
Approved

Last updated: December 12, 2025View editorial policy

Personalize

Help us tailor your experience

Which best describes you? Your choice helps us use language that's most understandable for you.

What is 250 ml SF 0.9%?

250 ml SF 0.9% refers to 250 milliliters of 0.9% sodium chloride solution (normal saline), which is an isotonic crystalloid fluid containing 154 mEq/L of both sodium and chloride.

Composition and Classification

  • 0.9% NaCl (normal saline) is an isotonic crystalloid solution that contains 9 grams of sodium chloride per liter, equivalent to 154 mEq/L of sodium and 154 mEq/L of chloride 1.

  • This solution has an osmolality of approximately 308 mOsm/kg, making it isotonic with plasma 1.

  • Normal saline is classified as a non-balanced crystalloid due to its supraphysiologic chloride content compared to plasma (154 mEq/L vs. 98-106 mEq/L physiologically) 1.

Clinical Applications

  • European trauma guidelines recommend crystalloids like 0.9% NaCl as initial fluid therapy for bleeding trauma patients (Grade 1B recommendation) 1.

  • In diabetic ketoacidosis (DKA), isotonic saline (0.9% NaCl) is the recommended initial fluid at 15-20 ml/kg/h during the first hour for intravascular volume expansion and restoration of renal perfusion 1.

  • For surgical patients, 250 ml volumes of crystalloid can be used for fluid responsiveness testing, with changes in stroke volume index predicting the effects of larger fluid boluses 2.

Important Clinical Considerations

Hyperchloremia Risk

  • Administration of large volumes of 0.9% NaCl (>5000 mL) is associated with hyperchloremia, which has been linked to increased mortality in observational studies of high-risk surgical and ICU patients 1.

  • Balanced crystalloids (such as Ringer's Lactate or Plasmalyte) are probably preferred over 0.9% NaCl for hemorrhagic shock resuscitation to reduce mortality and adverse renal events (Grade 2+ recommendation) 1.

Volume Distribution

  • Crystalloids like 0.9% NaCl distribute throughout the extracellular space, with only approximately 25-30% remaining in the intravascular compartment after equilibration 1.

  • The volume expansion capacity of crystalloids is lower than colloids (mean ratio of 1:1.5), requiring larger volumes to achieve similar hemodynamic effects 1.

Contraindications and Cautions

  • Avoid 0.9% NaCl as primary fluid in hypernatremic dehydration, as it paradoxically worsens hypernatremia by providing excessive osmotic load 3.

  • In patients requiring large-volume resuscitation, monitor for hyperchloremic metabolic acidosis, which can complicate acid-base interpretation and potentially worsen renal function 1.

  • Patients with cardiac or renal compromise require more frequent monitoring during fluid administration to avoid iatrogenic fluid overload 4, 3.

References

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Guideline

Calculating Water Deficit and D5W Requirements for Hypernatremia

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Guideline

Mixing 3% Normal Saline with Potassium Chloride

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

Have a follow-up question?

Our Medical A.I. is used by practicing medical doctors at top research institutions around the world. Ask any follow up question and get world-class guideline-backed answers instantly.