What is the difference between PD-L1 (Programmed Death-Ligand 1) and PD-1 (Programmed Death-1) inhibitors?

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Last updated: December 13, 2025View editorial policy

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Difference Between PD-1 and PD-L1 Inhibitors

PD-1 inhibitors block the receptor on immune cells while PD-L1 inhibitors block the ligand on tumor cells, and PD-1 inhibitors cause more immune-related adverse events—particularly pneumonitis and hypothyroidism—compared to PD-L1 inhibitors, though both achieve similar efficacy in cancer treatment. 1

Molecular Mechanism and Target Location

PD-1 Inhibitors

  • Target the receptor on activated immune cells, specifically T cells, preventing the inhibitory signal that would normally suppress T cell function 2, 3
  • Block PD-1 expressed on the surface of T cells and other activated immune cells 2
  • FDA-approved agents include nivolumab (Opdivo) and pembrolizumab (Keytruda) 2, 4

PD-L1 Inhibitors

  • Target the ligand expressed on tumor cells and antigen-presenting cells in the tumor microenvironment 2, 5
  • Block PD-L1 on tumor cells, preventing it from binding to PD-1 receptors 5, 6
  • FDA-approved agents include atezolizumab (Tecentriq), durvalumab (Imfinzi), and avelumab (Bavencio) 2, 4

Functional Outcome

  • Both approaches achieve the same end result: disrupting the PD-1/PD-L1 interaction to restore T cell-mediated tumor killing 2, 5
  • The blockade prevents tumor cells from evading immune surveillance and promotes T cell activation 2, 3

Safety Profile Differences

PD-1 Inhibitors Have Higher Toxicity

  • Overall immune-related adverse events (irAEs) trend higher with PD-1 inhibitors: 16% versus 11% for PD-L1 inhibitors (P=0.07) 1
  • Pneumonitis occurs twice as frequently with PD-1 inhibitors: 4% versus 2% (P=0.01) for any-grade, and 3.6% versus 1.3% (P=0.001) in another meta-analysis 1
  • High-grade pneumonitis is also more common: 1.1% versus 0.4% (P=0.02) 1
  • Hypothyroidism is more frequent with PD-1 inhibitors: 6.7% versus 4.2% (P=0.07) 1

Severe Toxicity Rates Are Similar

  • The incidence of severe (high-grade) irAEs is not significantly different: 5% for PD-1 versus 3% for PD-L1 inhibitors (P=0.4) 1
  • Both classes can cause vitiligo, thyroid dysfunction, hepatotoxicity, and pneumonitis, though frequencies differ 1

Clinical Implication

  • Monitor more closely for pneumonitis when using PD-1 inhibitors, especially in patients with non-small cell lung cancer or pre-existing lung disease 1
  • The safety data for PD-L1 inhibitors are still maturing, with ongoing data collection 1

Clinical Efficacy Considerations

Biomarker Testing

  • PD-L1 expression testing is recommended for all patients with newly diagnosed advanced NSCLC to guide treatment decisions 2, 5
  • Testing uses immunohistochemistry assays evaluating membranous immunostaining of tumor cells 2, 5
  • PD-L1 is not a perfect biomarker: less than half of biomarker-selected patients benefit, and some responses occur in biomarker-negative cohorts 5

Treatment Selection

  • Either PD-1 or PD-L1 inhibitors can be selected based on FDA approvals for specific cancer types, as both block the same pathway 2, 4
  • The choice between PD-1 and PD-L1 inhibitors should factor in the lower pneumonitis risk with PD-L1 agents when treating patients with baseline lung compromise 1

Important Clinical Pitfalls

Infectious Complications

  • Both PD-1 and PD-L1 blockade may reactivate latent tuberculosis and other opportunistic infections, requiring screening in high-risk patients 1
  • The immune modulation can unmask chronic underlying infections with detrimental effects on outcomes 1

Combination Therapy Toxicity

  • Combined PD-1 plus CTLA-4 blockade causes substantially more irAEs than PD-1 monotherapy: 55-60% high-grade events versus 10-20% 1
  • Treatment discontinuation due to adverse events occurs in 39% with combination therapy versus 12% with PD-1 monotherapy 1

Tumor-Specific Considerations

  • In melanoma patients treated with PD-1 inhibitors, higher incidence of GI and skin irAEs but lower pneumonitis compared to NSCLC patients 1
  • Renal cell carcinoma patients experience higher frequency of pneumonitis and dyspnea compared to melanoma patients 1

References

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Guideline

PD-1/PD-L1 Axis in Cancer Immunotherapy

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Research

PD-L1: From cancer immunotherapy to therapeutic implications in multiple disorders.

Molecular therapy : the journal of the American Society of Gene Therapy, 2024

Guideline

PD-L1 Expression and Immunotherapy

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Research

PD-L1.

Journal of clinical pathology, 2018

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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