What is RAPD (Rapid Amplification of Polymorphic DNA)?

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What is RAPD (Relative Afferent Pupillary Defect)

RAPD is a relative afferent pupillary defect—an asymmetric pupillary response to light indicating unilateral or asymmetric optic nerve dysfunction, retinal disease, or other afferent visual pathway pathology. 1

Clinical Definition and Mechanism

  • RAPD represents impaired afferent input from one eye compared to the fellow eye, detected through the swinging flashlight test where light directed at the affected eye produces less pupillary constriction than when directed at the normal eye 1

  • The pupils themselves are structurally normal; the defect lies in the afferent visual pathway (retina, optic nerve, or optic tract) rather than the efferent pupillomotor pathway 1

Clinical Significance and Measurement

  • An RAPD of 0.3 or more log units (easily visible clinically) is NOT typically seen in amblyopia alone, making it a critical distinguishing feature 1

  • A subtle RAPD may occur with dense amblyopia, but the presence of a large RAPD should prompt immediate investigation for compressive optic neuropathy or significant retinal pathology 1

Diagnostic Approach in Pediatric Patients

  • Pupillary examination should assess size, symmetry, shape, direct and consensual light responses, and presence of relative afferent defect 1

  • Testing in infants and children may be challenging due to hippus (physiologic pupillary oscillation), poorly maintained fixation, and rapid accommodative changes 1

  • Anisocoria greater than 1 millimeter warrants evaluation for pathological processes including Horner syndrome, Adie tonic pupil, or third-cranial-nerve palsy 1

Critical Clinical Pitfall

  • The presence of a large RAPD mandates a thorough search for compressive optic neuropathy or other etiologies of visual impairment such as significant retinal abnormalities, optic neuritis, or ischemic optic neuropathy 1

  • Irregular pupils may indicate traumatic sphincter damage, iritis, or congenital abnormalities like coloboma, which should be distinguished from RAPD 1

References

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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