From the Guidelines
HBSS stands for Hanks' Balanced Salt Solution, not a genetic abbreviation related to a disease. This is based on the most recent evidence from the 2020 international consensus on first aid science with treatment recommendations 1. The evidence clearly indicates that HBSS is a storage solution, often used in the context of storing avulsed teeth, and has been shown to have benefits in maintaining tooth cell viability compared to other storage media, such as milk 1. Key points about HBSS include:
- It is a balanced salt solution used for storing avulsed teeth
- It has been shown to have a positive effect on tooth cell viability compared to milk
- It is recommended as a storage solution in the 2020 international consensus on first aid science with treatment recommendations, along with other solutions like propolis, oral rehydration salts, and cling film 1. In the context of first aid and emergency medicine, using HBSS as a storage solution for avulsed teeth is a recommended practice, as it can help improve outcomes by maintaining the viability of periodontal ligament cells, which is crucial for successful tooth replantation 1.
From the Research
Definition of HBSS
- HBSS stands for Hank's Balanced Salt Solution, a buffered salt solution used for a variety of cell culture applications 2, 3, 4, 5, 6.
Composition and Use
- It is a mixture of salts, sugars, and other compounds that provides a nutrient-rich environment for cells to grow and thrive 2, 3, 4.
- HBSS is commonly used as a storage medium for avulsed teeth, as it helps to maintain the viability of periodontal ligament fibroblasts (PDLF) and other cells 2, 3, 4.
- It is also used as a transport medium for cells and tissues, and as a buffer solution for various biochemical assays 5, 6.
Comparison with Other Media
- HBSS has been compared to other storage media, such as Dulbecco's Modified Eagle Medium (DMEM), normal saline, and coconut water, in terms of its ability to maintain cell viability 2, 3, 5.
- Studies have shown that HBSS is effective in maintaining PDLF viability, but its effectiveness can be improved by the addition of supplements such as vitamin C or platelet-derived growth factor 2, 3.
- HBSS has also been compared to other balanced salt solutions, such as Krebs-Ringer balanced salt solution, in terms of its ability to support the survival of polymorphonuclear leukocytes (PMNs) and other cells 6.