Can buspirone (anxiolytic medication) cause a false positive result for amphetamine on a drug screen?

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Can Buspirone Cause a False-Positive Amphetamine Screen?

No, buspirone is not documented in the medical literature as a cause of false-positive amphetamine urine drug screens.

Evidence Review

The available evidence does not identify buspirone among the medications known to cause false-positive amphetamine results. Multiple comprehensive reviews of cross-reacting substances have been conducted, and buspirone does not appear in these analyses 1, 2, 3.

Documented Causes of False-Positive Amphetamine Screens

The following medications ARE known to cause false-positive amphetamine results and should be considered in your differential:

  • Bupropion is the most frequent cause of false-positive amphetamine screens, accounting for 41% of unconfirmed positive results in one large study 4
  • Trazodone causes false positives through cross-reactivity of its metabolite m-CPP with amphetamine immunoassays 5
  • Aripiprazole has been documented in pediatric case reports to cause false-positive amphetamine screens 6
  • Pseudoephedrine in over-the-counter cold medications commonly causes false-positive amphetamine/methamphetamine results 7
  • Other antidepressants, antipsychotics, labetalol, fenofibrate, and metformin have also been implicated 3

Critical Clinical Approach

When encountering a positive amphetamine screen in a patient denying use:

  • Obtain a complete medication history including all prescription medications, over-the-counter drugs, and supplements before interpreting results 7, 5
  • Order confirmatory testing with gas chromatography-mass spectrometry (GC-MS) immediately before making any clinical decisions, as immunoassay screening tests are presumptive only 7, 5, 6
  • Never make punitive decisions based solely on immunoassay results without confirmatory testing 5
  • Discuss unexpected results with laboratory personnel or toxicologists before taking action 7, 8

Important Caveats

  • False-positive amphetamine screens occur in 3.9-9.9% of positive amphetamine immunoassays in clinical laboratories 2
  • Many providers have inadequate training in interpreting urine drug test results, and incorrect interpretation can have severe consequences including legal implications 9, 7
  • Establishing a working relationship with your local toxicologist is recommended for consultation on complex results 9, 5

References

Research

False-Positive Amphetamines in Urine Drug Screens: A 6-Year Review.

Journal of analytical toxicology, 2023

Research

Frequency of false positive amphetamine screens due to bupropion using the Syva EMIT II immunoassay.

Journal of medical toxicology : official journal of the American College of Medical Toxicology, 2011

Guideline

Trazodone-Associated False Positives in Drug Screens

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Guideline

Interpreting Urine Drug Test Results

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Guideline

Limitations of Standard Urine Drug Tests

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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