What is the recommended dose of tenecteplase (TNK-tPA) for myocardial infarction (MI)?

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Tenecteplase Dosing for Myocardial Infarction

Tenecteplase should be administered as a single intravenous bolus using weight-based dosing: 30 mg for patients <60 kg, 35 mg for 60-<70 kg, 40 mg for 70-<80 kg, 45 mg for 80-<90 kg, and 50 mg for patients ≥90 kg. 1, 2, 3

Weight-Based Dosing Table

The following weight-tiered dosing regimen is recommended:

Patient Weight Tenecteplase Dose Volume to Administer
<60 kg 30 mg 6 mL
60-<70 kg 35 mg 7 mL
70-<80 kg 40 mg 8 mL
80-<90 kg 45 mg 9 mL
≥90 kg 50 mg 10 mL

1, 2, 4, 3

Administration Details

  • Tenecteplase is given as a single intravenous bolus over 5-10 seconds, which represents a major practical advantage over alteplase's 90-minute infusion. 3, 5

  • The drug should be reconstituted with 10 mL of Sterile Water for Injection to achieve a final concentration of 5 mg/mL. 3

  • Weight-adjusted dosing is critical because total body weight explains 19% of the variability in plasma clearance—a 10 kg increase in body weight results in a 9.6 mL/min increase in clearance. 6, 7

Timing Requirements

  • Tenecteplase must be administered within 12 hours of symptom onset, with greatest benefit occurring with earlier administration. 1, 2

  • The primary indication is when primary PCI cannot be performed by an experienced team within 120 minutes of first medical contact. 8, 1, 2

  • For patients presenting very early (<2 hours) with large infarcts and low bleeding risk, consider fibrinolysis if time from first medical contact to balloon inflation exceeds 90 minutes. 1, 2, 4

Mandatory Adjunctive Antiplatelet Therapy

Aspirin:

  • Loading dose: 150-500 mg orally or 250 mg IV if oral route not possible 1, 2
  • Maintenance: 75-100 mg daily indefinitely 1, 4

Clopidogrel:

  • Loading dose: 300 mg orally for patients ≤75 years 1, 4
  • No loading dose for patients >75 years—start with 75 mg daily 1
  • Maintenance: 75 mg daily for minimum 14 days 4

Note that prasugrel and ticagrelor have not been studied as adjuncts to fibrinolysis and should not be given. 8

Required Anticoagulation

Enoxaparin (preferred over unfractionated heparin):

  • Patients <75 years: 30 mg IV bolus, then 1 mg/kg subcutaneous every 12 hours (maximum 100 mg for first two doses) 1, 4

  • Patients ≥75 years: No IV bolus; start with 0.75 mg/kg subcutaneous every 12 hours (maximum 75 mg for first two doses) 1, 4

  • Renal impairment (creatinine clearance <30 mL/min): Subcutaneous doses given once every 24 hours regardless of age 1

  • Duration: Until revascularization or for hospital stay up to 8 days 8, 1, 4

Unfractionated heparin (alternative):

  • 60 U/kg IV bolus (maximum 4000 U) followed by 12 U/kg/hour infusion (maximum 1000 U/hour) for 24-48 hours 1, 4

  • Target aPTT: 50-70 seconds or 1.5-2.0 times control, monitored at 3,6,12, and 24 hours 1

  • Careful monitoring is mandatory—aPTT values >70 seconds are associated with higher likelihood of bleeding, reinfarction, and death. 8

Post-Administration Monitoring and Management

  • Monitor ST-segment elevation, cardiac rhythm, and clinical symptoms over 60-180 minutes after administration. 1, 2

  • Signs of successful reperfusion include relief of symptoms, hemodynamic/electrical stability, and ≥50% reduction of initial ST-segment elevation on follow-up ECG at 60-90 minutes. 1, 2, 4

  • All patients must be transferred to a PCI-capable center following fibrinolysis. 8, 1, 2, 4

  • Rescue PCI is indicated immediately if fibrinolysis fails (<50% ST-segment resolution at 60 minutes). 8, 1, 2

  • For stable patients after successful fibrinolysis, routine angiography with view to revascularization should occur at 3-24 hours. 1

Pharmacologic Rationale

  • Tenecteplase has 15-fold higher fibrin specificity, 80-fold reduced binding to PAI-1, and 6-fold prolonged plasma half-life (22 minutes initial, 90-130 minutes terminal) compared to alteplase. 9, 6, 7

  • The biphasic elimination allows for single bolus administration with mean plasma clearance of 99-119 mL/min across the 30-50 mg dose range. 3, 6, 7

  • Tenecteplase achieves 85% TIMI 2-3 flow at 90 minutes, with more than 75% of patients achieving normal flow when plasma AUC exceeds 320 μg·min/mL. 4, 6

Clinical Efficacy Evidence

  • The ASSENT-2 trial (16,949 patients) demonstrated equivalent 30-day mortality between tenecteplase and alteplase (6.2% for both), with similar rates of intracranial hemorrhage (0.9% vs 0.9%) and stroke (1.8% vs 1.7%). 3, 9

  • Tenecteplase showed reduced mortality in patients receiving treatment more than 4 hours after symptom onset (7% vs 9.2%, p=0.018) and lower rates of non-cerebral bleeding (26.4% vs 29.0%, p=0.0003). 9, 5

Critical Safety Considerations

  • Avoid tenecteplase in patients >75 years when using enoxaparin as ancillary therapy due to increased intracranial hemorrhage risk demonstrated in ASSENT-3 PLUS. 8

  • Avoid low molecular weight heparin in patients with significant renal dysfunction (serum creatinine >2.5 mg/dL in men or >2.0 mg/dL in women). 2

  • The risk of intracranial hemorrhage is 0.9-1.0% overall, increased with lower weight, female sex, previous cerebrovascular disease, and hypertension on admission. 4

References

Guideline

Tenecteplase Dosing and Administration for Acute Myocardial Infarction

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Guideline

Administration Protocol for Tenecteplase in Myocardial Infarction

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Guideline

Thrombolytic Dosing for Myocardial Infarction

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Research

Tenecteplase: a review of its pharmacology and therapeutic efficacy in patients with acute myocardial infarction.

American journal of cardiovascular drugs : drugs, devices, and other interventions, 2001

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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