What are the dosing guidelines for Intravenous (IV) Biotin repletion?

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Last updated: December 21, 2025View editorial policy

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IV Biotin Dosing Guidelines

For IV biotin repletion, administer up to 200 mg/day for 2-3 weeks in parenteral nutrition-dependent patients with documented biotin deficiency. 1

Route Selection Algorithm

IV biotin is reserved exclusively for specific clinical scenarios—you should NOT use IV biotin unless the patient meets these criteria:

  • Parenteral nutrition (PN)-dependent patients who cannot receive enteral/oral nutrition 1
  • Patients with complete intestinal failure requiring total PN 1

Oral biotin is first-line for all other scenarios, including malabsorption and short bowel syndrome, where doses up to 10 mg/day orally are recommended rather than IV administration 1. This is a critical distinction—even severe malabsorption does not automatically warrant IV therapy.

IV Dosing Regimens

Treatment of Deficiency (PN-Dependent Patients)

  • Acute repletion dose: Up to 200 mg/day IV for 2-3 weeks 1
  • This high-dose regimen is supported by ESPEN guidelines specifically for PN-dependent patients with documented deficiency 1

Maintenance Dosing in Parenteral Nutrition

  • Standard PN maintenance: 60 mcg/day IV (Grade B recommendation) 1
  • This represents the baseline requirement for patients on long-term PN 1
  • One case report demonstrated successful maintenance with 60 mcg/day after initial repletion with 5 mg/day 2

Special Considerations

Renal Replacement Therapy

  • Patients on dialysis may require additional biotin supplementation beyond standard PN doses due to dialytic losses 1
  • Specific dosing adjustments are not well-established but should be considered 1

Safety Profile

  • No upper limit has been established for biotin intake 1, 3
  • No adverse effects reported with IV doses up to 5 mg/day for prolonged periods 1, 3
  • The 200 mg/day treatment dose is well-tolerated for short-term (2-3 week) repletion 1

Critical Pitfalls to Avoid

Biotin interference with laboratory assays: High-dose biotin (>5 mg/day) interferes with streptavidin-biotin immunoassays, causing falsely abnormal results for troponin, thyroid function tests, beta-hCG, and tumor markers 4. Patients should abstain from biotin for 48 hours before critical laboratory testing 4.

Inappropriate IV use: Do not default to IV biotin for convenience in patients with functional GI tracts—oral absorption is nearly complete even in malabsorption states, though higher oral doses (10 mg/day) may be needed 1, 5.

References

Guideline

Biotin Administration Guidelines

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Research

Biotin deficiency in a patient with short bowel syndrome during home parenteral nutrition.

JPEN. Journal of parenteral and enteral nutrition, 1984

Guideline

Biotin Interference with Thyroid Function Tests

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Research

Biotin biochemistry and human requirements.

The Journal of nutritional biochemistry, 1999

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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