Would you recommend fecal calprotectin (FC) in the management of acute Shiga toxin-producing Escherichia coli (E. coli) infection?

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Fecal Calprotectin is NOT Recommended for Acute STEC Infection Management

Do not use fecal calprotectin to guide management decisions in acute Shiga toxin-producing E. coli (STEC) infections. The 2017 Infectious Diseases Society of America (IDSA) guidelines explicitly state there are insufficient data to recommend fecal calprotectin measurement in people with acute infectious diarrhea 1. This applies directly to STEC infections.

Why Calprotectin Has No Role in STEC Management

Limited Evidence in Acute Infectious Diarrhea

  • The IDSA guidelines found conflicting and limited reports about fecal calprotectin's value in acute infectious diarrhea, with some studies suggesting higher levels may indicate bacterial etiologies while others found no diagnostic value 1.

  • Fecal calprotectin is elevated in various inflammatory conditions including infectious gastroenteritis, making it non-specific for distinguishing STEC from other causes of acute diarrhea 2, 3.

  • The marker cannot differentiate between bacterial pathogens or guide specific treatment decisions in the acute infectious setting 1.

Focus on Critical STEC-Specific Monitoring

Instead of calprotectin, the IDSA strongly recommends frequent monitoring of hemoglobin, platelet counts, electrolytes, blood urea nitrogen, and creatinine to detect early manifestations of hemolytic uremic syndrome (HUS) in patients with diagnosed STEC infection 1. This is a strong recommendation based on high-quality evidence.

  • Examining a peripheral blood smear for red blood cell fragmentation is necessary when HUS is suspected 1.

  • This monitoring is especially critical for STEC producing Shiga toxin 2 or infections associated with bloody diarrhea 1.

Clinical Pitfalls to Avoid

  • Do not delay appropriate supportive care or HUS monitoring while waiting for calprotectin results in suspected STEC infection 1.

  • Antimicrobial therapy is contraindicated in STEC infections as it may increase Shiga toxin production and HUS risk 4, 5.

  • Serologic testing for STEC may be considered only for post-diarrheal HUS when stool culture did not yield a Shiga toxin-producing organism, but this is a weak recommendation 1.

When Calprotectin IS Useful (Not in STEC)

Fecal calprotectin has established utility in inflammatory bowel disease (IBD) for diagnosing, monitoring disease activity, and guiding treatment adjustments, with cutoffs >150 μg/g indicating active inflammation 1, 2. However, this application is entirely separate from acute infectious diarrhea management 1.

References

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Guideline

Calprotectin as a Biomarker for Intestinal Inflammation

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Guideline

Elevated Calprotectin Levels: Causes and Next Steps

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Research

Shiga toxin-producing Escherichia coli (STEC).

Clinics in laboratory medicine, 2010

Research

Treatment Strategies for Infections With Shiga Toxin-Producing Escherichia coli.

Frontiers in cellular and infection microbiology, 2020

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Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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