What is the proper preparation method for Nutramigen (hydrolyzed protein formula)?

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Last updated: December 22, 2025View editorial policy

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Proper Preparation of Nutramigen

Nutramigen should be prepared according to standard powdered infant formula preparation guidelines: use one level scoop of powder (provided in the can) to 2 fluid ounces of water, mix thoroughly, and use immediately or refrigerate prepared formula at 2-8°C for no more than 24 hours.

Standard Preparation Instructions

  • Mix one unpacked, level scoop of Nutramigen powder with 2 fluid ounces (60 mL) of water to achieve the standard 20 calories per ounce dilution 1
  • Use clean, sanitized bottles and preparation surfaces to minimize bacterial contamination risk 1
  • Shake or stir the formula thoroughly until powder is completely dissolved before feeding 1

Formula Forms and Handling

Nutramigen is available in three forms, each with specific handling requirements 1:

  • Powder form: Requires mixing with water as described above; most economical but requires most manipulation and carries highest contamination risk 1
  • Liquid concentrate: Requires dilution with equal parts water; intermediate convenience 1
  • Ready-to-feed: No preparation needed; safest option with lowest contamination risk but most expensive 1

Storage and Safety Guidelines

  • Prepared formula must be used immediately or stored at 2-8°C (refrigerated) and used within 24 hours 1
  • Each manipulation step during formula preparation introduces potential bacterial contamination 1
  • Discard any formula left in the bottle after feeding; do not save for later use 1
  • Unopened powder cans should be stored in a cool, dry place 1

Clinical Context

Nutramigen is an extensively hydrolyzed casein formula designed for infants with cow's milk protein allergy 2, 3. The formula has demonstrated:

  • 80-90% success rate in managing cow's milk protein allergy when used as first-line treatment in formula-fed infants 4
  • Well-tolerated with appropriate growth parameters in infants with confirmed cow's milk allergy 5
  • No allergic reactions observed in controlled studies of infants with cow's milk allergy 5

Critical Preparation Pitfalls to Avoid

  • Never use more or less powder than specified (1 scoop per 2 oz water), as this alters caloric density and osmolality, potentially causing feeding intolerance or inadequate nutrition 1
  • Never use unpurified or contaminated water sources; use sterile or boiled-then-cooled water for immunocompromised or very young infants 1
  • Never prepare formula in advance for more than 24 hours, as this increases bacterial growth risk 1
  • Do not pack or heap the scoop; use only a level scoop to ensure accurate concentration 1

Special Considerations

  • Nutramigen contains maize-derived ingredients (corn starch) that may contain trace zein proteins, though clinical relevance remains unclear 6
  • The formula is lactose-free and suitable for infants requiring lactose restriction 7
  • Individual formula recipes and dilution instructions vary by product, so always verify specific mixing ratios on the product label 1

References

Research

What's in the Bottle? A Review of Infant Formulas.

Nutrition in clinical practice : official publication of the American Society for Parenteral and Enteral Nutrition, 2016

Research

Comparison of a partially hydrolyzed infant formula with two extensively hydrolyzed formulas for allergy prevention: a prospective, randomized study.

Pediatric allergy and immunology : official publication of the European Society of Pediatric Allergy and Immunology, 2000

Research

Preventive effect of feeding high-risk infants a casein hydrolysate formula or an ultrafiltrated whey hydrolysate formula. A prospective, randomized, comparative clinical study.

Pediatric allergy and immunology : official publication of the European Society of Pediatric Allergy and Immunology, 1993

Guideline

Cow Milk Protein Allergy Management in Neonates

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Research

Identification of immunogenic maize proteins in a casein hydrolysate formula.

Pediatric allergy and immunology : official publication of the European Society of Pediatric Allergy and Immunology, 2000

Guideline

Lactose-Free Infant Formula Guidelines

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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