Can B1 (Thiamine), B6 (Pyridoxine), and B12 (Cobalamin) supplements cause dark stools?

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Last updated: December 22, 2025View editorial policy

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Can B1, B6, and B12 Supplements Cause Dark Stool?

B1 (thiamine), B6 (pyridoxine), and B12 (cobalamin) supplements do not cause dark stools as a recognized side effect. These water-soluble vitamins are not associated with stool color changes in clinical guidelines or pharmacological literature.

Known Side Effects of B-Vitamin Supplementation

Thiamine (Vitamin B1)

  • No gastrointestinal effects on stool color are documented 1
  • High IV doses (>400 mg) may rarely induce nausea, anorexia, and mild ataxia, but not stool changes 2
  • Excess thiamine is excreted in urine without causing stool discoloration 2

Pyridoxine (Vitamin B6)

  • The primary concern with B6 is neuropathy and photosensitivity at excessive doses, not gastrointestinal effects 1
  • Doses above 100 mg/day can cause sensory neuropathy 1
  • No stool color changes are listed among adverse effects in clinical guidelines 1
  • Vitamin B6 overload from supplementation can cause polyneuropathy but does not affect stool appearance 3, 4

Cobalamin (Vitamin B12)

  • No known adverse effects on stool color or appearance 1
  • The guideline literature lists "unknown" for effects of excess B12 1
  • B12 deficiency can cause pernicious anemia and neurologic deterioration, but supplementation does not alter stool color 1

Important Clinical Distinction

Dark or black stools warrant immediate medical evaluation for gastrointestinal bleeding, which appears as melena (tarry, black stools) and represents a potentially serious condition unrelated to B-vitamin supplementation.

Alternative Causes of Dark Stools to Consider:

  • Iron supplementation is a well-documented cause of dark or black stools 1
  • Bismuth-containing medications (e.g., Pepto-Bismol)
  • Foods with dark pigments (blueberries, black licorice, dark leafy greens)
  • Gastrointestinal bleeding (requires urgent evaluation)

Clinical Recommendation

If a patient taking B1, B6, and B12 supplements develops dark stools, investigate other causes rather than attributing it to the B vitamins. Check for concurrent iron supplementation, dietary factors, or signs of gastrointestinal bleeding. The B-vitamin complex itself is not responsible for stool color changes based on established pharmacological profiles 1.

References

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Guideline

Thiamine Supplementation Guidelines

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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