What causes gallstones?

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What Causes Gallstones?

Gallstones form when there is an imbalance in the composition of bile, resulting in precipitation of one or more of its components—primarily cholesterol supersaturation, but also bilirubin and other bile constituents. 1

Primary Mechanisms of Gallstone Formation

Cholesterol Gallstones (Most Common Type)

Cholesterol gallstones develop when bile becomes supersaturated with cholesterol due to increased hepatic cholesterol synthesis and secretion into bile. 2 This represents 37-86% of all gallstones and is the predominant type in Europe and North America. 1

The key pathophysiologic mechanisms include:

  • Increased cholesterol secretion by the liver into bile, exceeding the solubilizing capacity of bile acids and phospholipids 2
  • Decreased bile acid synthesis or secretion, reducing the ability to keep cholesterol in solution 2
  • Impaired gallbladder motility, allowing cholesterol crystals to nucleate and grow rather than being expelled 3

Pigment Stones (Less Common)

Black pigment stones form due to chronic hemolysis, which increases unconjugated bilirubin in bile that precipitates as calcium bilirubinate. 4

Brown pigment stones typically develop in obstructed and infected bile ducts, where bacterial enzymes deconjugate bilirubin. 4

Major Risk Factors

Non-Modifiable Risk Factors

  • Female sex: Women have higher prevalence due to estrogen and progesterone exposure, which increases cholesterol secretion and decreases gallbladder motility 1, 3
  • Age: Gallstone disease increases progressively with age 3, 1
  • Genetics: Common mutations in the hepatic cholesterol transporter ABCG8 (particularly the p.D19H variant) confer approximately 25% of total gallstone risk 4, 5
  • Pregnancy: Hormonal changes lead to decreased gallbladder motility and lithogenic bile 3

Modifiable Risk Factors

Obesity is a major risk factor, with those having the highest body mass index showing 5-6 times increased relative risk. 6 This is attributed to:

  • Increased hepatic cholesterol synthesis and secretion 6
  • Diets high in refined carbohydrates and saturated fat 1, 7

Rapid weight loss paradoxically increases gallstone risk, with new stone formation occurring in 10-12% after 8-16 weeks of low-calorie diet and >30% within 12-18 months after gastric bypass surgery. 6 Risk factors during weight loss include:

  • Weight loss >24% of initial body weight 6
  • Rate of weight loss >1.5 kg per week 6
  • Very low-calorie diets with no fat 6
  • Long overnight fasting periods 6

Physical inactivity and metabolic syndrome components increase gallstone risk. 4

Specific Medical Conditions

  • Chronic liver disease and cirrhosis are associated with pigment stone formation 3
  • Chronic hemolytic conditions (e.g., sickle cell disease) cause black pigment stones 4
  • Primary sclerosing cholangitis increases risk of cholangiocarcinoma and gallbladder cancer 3
  • Inflammatory bowel disease is associated with increased gallstone risk 3

Genetic Disorders

Low phospholipid-associated cholelithiasis syndrome results from loss-of-function mutations in the ABCB4 transporter, causing low phosphatidylcholine concentrations in bile. 5 This represents a smaller subset of patients with primarily genetic gallstone disease. 5

ABCB11 (BSEP) mutations can cause progressive familial intrahepatic cholestasis with associated gallstone formation. 3

Clinical Implications

Approximately 80% of patients with gallstones remain asymptomatic throughout their lifetime, with only 1-4% developing symptoms annually. 1, 8 The likelihood of developing symptoms actually diminishes over time in those who remain asymptomatic. 1

Common Pitfalls to Avoid

  • Do not attribute non-specific symptoms like belching, bloating, fatty food intolerance, or chronic pain to gallstones—these are NOT caused by gallstone disease itself 9
  • Recognize that stone recurrence is common: Up to 50% of patients experience stone recurrence within 5 years after medical dissolution therapy with ursodiol 2
  • Understand that calcified stones and stones >20 mm rarely dissolve with medical therapy and require surgical management 2

References

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Research

Gallstones.

Nature reviews. Disease primers, 2016

Research

Genetics of gallstone disease.

European journal of clinical investigation, 2018

Research

Gallstones in obesity and weight loss.

European journal of gastroenterology & hepatology, 2000

Research

Nutritional approaches to prevention and treatment of gallstones.

Alternative medicine review : a journal of clinical therapeutic, 2009

Guideline

Asymptomatic and Symptomatic Gallstone Conditions

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Guideline

Gallstone Disease and Its Complications

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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