What is the recommended dose and injection site for Botox (Botulinum Toxin) in treating blepharospasm?

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Botox Dosing and Injection Sites for Blepharospasm

For blepharospasm, inject botulinum toxin into the pretarsal portion of the orbicularis oculi muscle using 1.25-2.5 U per site, with a typical total dose of 25-50 U per eye (12.5-25 U per side distributed across multiple injection points in both upper and lower eyelids). 1, 2

Injection Technique

Site Selection

  • Target the pretarsal portion of the orbicularis oculi muscle rather than the preseptal region, as this produces significantly higher response rates (95% vs 81% success), longer duration of benefit (12.5 weeks vs 8.5 weeks), and fewer complications like ptosis 2
  • Inject both upper and lower eyelids bilaterally 3, 4
  • Upper lid: Place 2 injections into the pretarsal orbicularis oculi 2
  • Lower lid: Place 1 injection into the pretarsal region 2
  • Additional sites may include the brow and upper face if spasms extend beyond the periocular region 3

Dosing Parameters

  • Standard starting dose: 12.5 U per side (25 U total per session) distributed across injection sites 4
  • Typical range: 25-50 U total per treatment session 3
  • For refractory cases requiring injections every 2 months despite standard dosing, supramaximal doses above 100 U per session may be considered and are well tolerated, potentially extending the interval between treatments 5

Expected Outcomes

Efficacy Timeline

  • Maximum effect occurs within 5 days of injection 3
  • Mean duration of benefit: 11.8-12.5 weeks with pretarsal technique 2, 3
  • Repeated injections maintain similar efficacy to initial treatment 3

Treatment Response

  • Success rate with pretarsal technique: 95% 2
  • All patients typically receive some degree of relief 4
  • Note that spasms may recur despite persistent orbicularis weakness, requiring repeat treatment 4

Safety Considerations

Common Adverse Effects

  • Ptosis occurs significantly less frequently with pretarsal injections compared to preseptal technique 2
  • Complications are local, mild, and transient 3
  • No systemic side effects have been reported in clinical trials 3
  • Dysphagia and aspiration risk can occur if toxin diffuses to swallowing muscles, particularly in patients with pre-existing neuromuscular disorders 6

Important Caveats

  • OnaBoNT-A and incoBoNT-A are equally effective and should be considered for blepharospasm treatment, while AboBoNT-A is a possibly effective option 7
  • Patients with combined blepharospasm and involuntary levator palpebrae inhibition respond better to pretarsal injection technique 2
  • Most side effects are temporary and resolve within 3-6 months as the toxin effect diminishes 6

References

Research

Pretarsal application of botulinum toxin for treatment of blepharospasm.

Journal of neurology, neurosurgery, and psychiatry, 1995

Research

Botulinum toxin in the management of blepharospasm.

Archives of neurology, 1986

Research

The use of botulinum toxin in blepharospasm.

American journal of ophthalmology, 1985

Guideline

Complications of DAO Botox Injections

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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