Bio Aesthetic Injection Points
For bio aesthetic procedures using autologous platelet concentrates (APCs) and hyaluronic acid fillers, inject using specific anatomical zones with precise needle gauges and depths to maximize safety and efficacy. 1
Facial Rejuvenation Injection Points
Intradermal Papule Technique for Fine Lines and Wrinkles
- Target areas: Crow's feet, glabellar lines, nasolabial folds, perioral wrinkles 1
- Needle specification: 30-gauge, 4mm length needles 1
- Injection depth: Intradermal layer only, forming visible papules with blanching to confirm proper placement 1
- Spacing: Inject 5mm apart from one another 1
- Bevel orientation: Face the bevel upward for optimal papule formation 1
- Treatment frequency: Sessions spaced at least one month apart for 3-4 sessions, then maintenance every 6-12 months 1
Microneedling Application Zones
- Forehead and glabella: 0.5-1.0mm depth 1
- Cheeks and malar areas: 0.5-1.5mm depth 1
- Nasolabial folds: 1.0-1.5mm depth 1
- Upper lip skin: 0.5-1.0mm depth 1
- Periorbital area (crow's feet): 0.25mm depth using lower settings for safety 1, 2
- Acne scars: Use stamping technique at greater depth for targeted treatment 1
Critical safety note: Never microneedle over tattoos or permanent makeup 1
Lip Augmentation Injection Points
Quadrant-Based Approach
- Anatomical division: Divide lips into four quadrants (two upper, two lower) 1, 3
- Needle technique: 27-30 gauge needles, retrograde injection fashion 1, 4
- Cannula technique: 25-gauge cannula with only two entry points for entire procedure 1, 4
- Injection depth: Above the orbicularis oris muscle (NOT orbicularis occuli as incorrectly stated in source) for vascular safety 1, 4, 3
- Volume per quadrant: 0.2-0.5 mL 1, 4
Critical anatomical landmarks to preserve: 3
- Vermilion border (white roll) - most critical aesthetic landmark
- Philtral columns as natural boundaries
- Sublabial concavity to avoid "duck lip" appearance
Periorbital Region Injection Points
Under-Eye and Crow's Feet Treatment
- Needle specification: 30-gauge for intradermal injection 2
- Cannula option: 22-gauge × 2 inches (~5cm) cannula for enhanced safety with Alb-PRF formulation 2
- Microneedling depth: 0.25mm when using device-based application 2
- Treatment protocol: Three sessions minimum, spaced 21 days apart, then maintenance every 6 months 2
Patient warning: Bruising and swelling are common in this delicate area 1, 2
Midface and Cheek Injection Points
Malar and Extended Midface Areas
- Primary zones: Left and right malar regions, extended midface 1, 5
- Intradermal injection: Monthly sessions for 3 areas (malar areas, nasolabial fold, upper lip skin) spaced 21 days apart 1
- Volume maintenance: Highest retention at 79.2% over 12 weeks in midface compared to other facial regions 5
Nasolabial Fold Specific Injection Points
Deep Fold Correction
- Injection approach: Target the fold directly with retrograde technique 1, 6
- Average volume: Slightly less than 1.5 mL per nasolabial fold for hyaluronic acid fillers 6
- Needle gauge: 27-30 gauge for precise placement 7
- Depth: Dermal to subdermal plane, avoiding superficial placement 1
Critical Safety Considerations Across All Injection Points
Vascular Complication Prevention
- Maintain injection depth above muscle layers to avoid encountering vessels within 1mm of injection site 3
- Use small gauge needles (30G) in delicate areas like periorbital region 1, 2
- Consider cannula technique to reduce bruising risk, particularly around lips and under-eyes 1, 2
Common Adverse Events to Warn Patients About
- Bruising: Typically resolves in 7-9 days 3
- Swelling and edema: Self-limited, resolves spontaneously 3, 2
- Tenderness: Mild to moderate, temporary 4
Post-Procedure Instructions
- Avoid sunlight for 24 hours post-procedure 1
- Avoid heavily scented facial products for 24 hours 1
- Maintain good home care routine and adequate sun protection to enhance results 2
Product-Specific Considerations
Autologous Platelet Concentrates (PRP/PRF)
- Timing constraint: PRF clots within 20-40 minutes if left in syringe; exposure to oxygen accelerates clotting 1
- Primary benefit: Rejuvenation and biostimulation rather than volumization 1, 4
- Duration: Rejuvenation effects persist 3 months; volumizing effect does not last beyond 3 months 1