What is the recommended time interval between 2 doses of Gardasil-9 (Human Papillomavirus 9-valent Vaccine)?

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Gardasil-9 Dosing Interval

For a 2-dose schedule of Gardasil-9, the second dose should be administered 6 months after the first dose. 1

Standard Dosing Schedules by Age

Two-Dose Schedule (Ages 9-14 years)

  • The CDC recommends a 2-dose schedule for individuals initiating vaccination before their 15th birthday 1
  • The second dose is administered 6 months after the first dose 2, 1
  • A 12-month interval between 2 doses produces a more robust immune response than a 6-month interval, though 6 months remains the standard recommendation 1
  • Geometric mean antibody titers are higher in the 9-14 year age group compared to older adolescents and young adults, supporting the 2-dose regimen 1

Three-Dose Schedule (Ages 15-26 years and older)

  • A 3-dose series is required for individuals initiating vaccination at age 15 years or older 1
  • Dose 2 should be given 1-2 months after dose 1 2, 1
  • Dose 3 should be administered 6 months after dose 1 2, 1
  • The minimum interval is 24 weeks between the first and third doses 1

Special Populations Requiring Three Doses

Immunocompromised Individuals

  • Persons with immunocompromising conditions require a 3-dose series regardless of age at initiation 1, 3
  • This includes individuals with HIV infection 2
  • The standard 3-dose schedule applies: 0,1-2 months, and 6 months 1

Important Considerations

Interrupted Schedules

  • If the vaccine series is interrupted, it does not need to be restarted 1
  • After interruption following the first dose, the second dose should be administered as soon as possible 1
  • A minimum interval of at least 12 weeks must be maintained between the second and third doses 1

Recent Updates

  • Recent Canadian guidance (2024) now supports a 1-dose schedule for ages 9-20 years, though this represents newer evidence not yet universally adopted 3
  • For individuals 21 years and older in the Canadian guidelines, a 2-dose schedule is recommended 3

Administration Details

  • Gardasil-9 is administered intramuscularly in the deltoid muscle or anterolateral thigh 4, 5
  • The vaccine can be given at the same visit as other age-appropriate vaccines using separate syringes at different anatomic sites 1

References

Guideline

HPV Vaccination Effectiveness and Recommendations

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Research

Summary of the National Advisory Committee on Immunization (NACI) Statement: Updated guidance on human papillomavirus (HPV) vaccines.

Canada communicable disease report = Releve des maladies transmissibles au Canada, 2024

Research

The human papillomavirus vaccines.

Acta dermatovenerologica Croatica : ADC, 2006

Research

Human papilloma virus vaccines: need to be introduced in India.

Human vaccines & immunotherapeutics, 2013

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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