What is the recommended course of action for an Rh-negative mother giving birth to an Rh-positive baby to prevent isoimmunization?

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Management of Rh-Negative Mother with Rh-Positive Baby

Give anti-D to the mother only—the baby does not receive anti-D prophylaxis. 1, 2

Rationale for Maternal-Only Administration

Anti-D immune globulin (RhIg) works by suppressing the maternal immune response to fetal Rh-positive red blood cells that enter maternal circulation during delivery. 2 The mechanism involves preventing the mother from developing antibodies that would attack Rh-positive red cells in future pregnancies, not treating the current infant. 2

  • The current baby is not at risk because this is typically the first exposure, and the mother has not yet developed anti-D antibodies. 1
  • Future pregnancies are the concern—without prophylaxis, the mother would develop antibodies that cause hemolytic disease of the fetus/newborn in subsequent Rh-positive pregnancies. 1

Specific Administration Protocol

Timing is critical:

  • Administer RhIg within 72 hours of delivery for optimal effectiveness. 1, 2
  • If the 72-hour window is missed, give RhIg as soon as recognized up to 28 days postpartum—delayed administration is less protective but still preferable to none. 1

Standard dosing:

  • Give 300 μg (1500 IU) intramuscularly or intravenously within 72 hours of delivering an Rh-positive infant. 1, 2
  • Alternatively, 120 μg may be used with testing for fetomaternal hemorrhage exceeding 6 mL of fetal red blood cells. 1

Additional Dose Requirements

Screen for excessive fetomaternal hemorrhage:

  • The standard 300 μg dose covers up to 15 mL of fetal red blood cells (approximately 30 mL of fetal blood). 1
  • For hemorrhage exceeding this amount, give an additional 10 μg of anti-D for every 0.5 mL of fetal red blood cells beyond the covered amount. 1, 3
  • Consider quantitative testing for fetomaternal hemorrhage following placental trauma, abruption, or abdominal trauma. 1, 3

Verification Requirements Before Administration

Confirm the following criteria are met: 2

  • Mother must be Rh-negative
  • Mother must not already be sensitized (no existing anti-D antibodies)
  • Baby must be Rh-positive
  • Baby should have a negative direct antiglobulin test

Critical Pitfall to Avoid

Do not administer anti-D to the infant—this is a maternal prophylaxis intervention only. The baby receives no benefit from anti-D administration and it is not indicated. 1, 2 The protection is entirely for future pregnancies by preventing maternal sensitization during the current delivery.

Effectiveness Data

Without postpartum RhIg, the rate of maternal sensitization is 12-13%. 2, 4 With proper postpartum administration within 72 hours, this drops to 1-2%. 1, 2, 4 When combined with the antenatal dose given at 28 weeks, sensitization rates decrease further to 0.1-0.2%. 1

References

Guideline

Management Guidelines for Rh Negative Pregnancy

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Research

Prevention of Rh alloimmunization.

Journal of obstetrics and gynaecology Canada : JOGC = Journal d'obstetrique et gynecologie du Canada : JOGC, 2003

Research

Anti-D administration after childbirth for preventing Rhesus alloimmunisation.

The Cochrane database of systematic reviews, 2000

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Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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