What are the next steps for a 57-year-old postmenopausal woman with elevated Cancer Antigen 125 (CA 125) levels?

Medical Advisory BoardAll articles are reviewed for accuracy by our Medical Advisory Board
Educational purpose only • Exercise caution as content is pending human review
Article Review Status
Submitted
Under Review
Approved

Last updated: January 14, 2026View editorial policy

Personalize

Help us tailor your experience

Which best describes you? Your choice helps us use language that's most understandable for you.

Elevated CA-125 in a 57-Year-Old Postmenopausal Woman

For a 57-year-old postmenopausal woman with elevated CA-125, immediate referral to a gynecologic oncologist is warranted, along with transvaginal ultrasound with color Doppler as the first-line imaging study to evaluate for ovarian malignancy. 1, 2

Initial Diagnostic Workup

Imaging Studies

  • Transvaginal ultrasound with color or power Doppler is the primary imaging modality and should be performed immediately to assess for ovarian masses, with specific attention to morphological features highly suggestive of malignancy including solid components, papillary projections, thick septations, ascites, or complex masses. 3, 2

  • The American College of Radiology O-RADS US system provides structured risk stratification: O-RADS 2 indicates <1% malignancy risk, O-RADS 3 indicates 1-10% risk, O-RADS 4 indicates 10-50% risk, and O-RADS 5 indicates ≥50% risk. 3

  • CT scan of abdomen and pelvis should be obtained as the first-line cross-sectional imaging study if ultrasound shows concerning features or is indeterminate. 4

  • MRI with IV contrast should be considered for further characterization if ultrasound findings are indeterminate or if there is concern about diaphragmatic involvement. 2, 4

Additional Tumor Markers

  • Measure serum CEA and CA 19-9 in addition to CA-125 to help distinguish primary ovarian tumors from gastrointestinal metastases. 3, 2

  • Calculate the CA-125/CEA ratio: if the ratio is ≥25, this favors ovarian origin; if ≤25, proceed with colonoscopy/gastroscopy to evaluate for gastrointestinal primary malignancy. 3, 2

Clinical Context and Interpretation

Why This Matters in Postmenopausal Women

  • Postmenopausal status significantly increases malignancy risk with elevated CA-125 compared to premenopausal women, with higher incidence of both gynecologic and non-gynecologic cancers. 5

  • The ACOG/SGO criteria specifically recommend referral for postmenopausal women with elevated CA-125, nodular or fixed pelvic mass, metastatic disease or ascites, or family history of breast or ovarian cancer. 1

  • In postmenopausal women without vaginal bleeding, the upper limit of normal for CA-125 should be 20 U/mL rather than the traditional 35 U/mL cutoff. 6

Sensitivity Limitations

  • CA-125 is elevated in approximately 85% of advanced epithelial ovarian cancers but only about 50% of early-stage cases, making imaging essential regardless of the CA-125 level. 3, 2

  • Do not rely on CA-125 alone to make surgical decisions, as it lacks specificity and can be elevated in numerous benign conditions. 3

Common Pitfalls and Benign Causes

Benign Conditions That Elevate CA-125

  • Endometriosis, pelvic inflammatory disease, ovarian cysts, uterine leiomyomas (especially with coexisting adenomyosis), and cirrhosis with ascites can all elevate CA-125, sometimes to very high levels (>1,000 U/mL). 3, 7, 8

  • Cirrhosis with ascites universally elevates CA-125 because mesothelial cells under pressure from fluid produce the antigen; do not test CA-125 in patients with ascites of any cause as it is universally elevated and nonspecific. 3

  • Previous radiotherapy may cause elevated CA-125 levels. 3

Critical Caveat

  • Serosal involvement (peritoneal, pleural, or pericardial fluid) requires cautious interpretation as normal epithelia produce CA-125 antigen, leading to false elevations in conditions like tuberculous peritonitis or pancreatic cancer. 9

Referral Criteria

Immediate gynecologic oncology referral is indicated for postmenopausal women meeting ACOG/SGO criteria, which includes any postmenopausal woman with elevated CA-125, particularly when combined with a pelvic mass on examination or imaging. 1, 4

Subspecialty consultation should be strongly considered before proceeding to surgery in postmenopausal patients with CA-125 >65 U/mL and an abdominopelvic mass, as approximately 74% of such cases represent gynecologic cancers at tertiary centers. 5

Non-Gynecologic Malignancies to Consider

  • Colorectal cancer and breast cancer can present with elevated CA-125 levels. 3

  • If CEA or CA 19-9 is elevated, especially with a CA-125/CEA ratio <25, endoscopy should be performed to evaluate for gastrointestinal primary malignancy. 3, 2

References

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Guideline

Investigation of Suspected Ovarian Cancer in Postmenopausal Women

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2026

Guideline

Cancers Associated with Elevated CA-125 Levels

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Guideline

Ovarian Cancer Risk Assessment with Elevated Inhibin B

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Research

Serum CA-125 measurements > 65 U/mL. Clinical value.

The Journal of reproductive medicine, 1997

Research

New reference levels for CA125 in pre- and postmenopausal women.

Primary care update for Ob/Gyns, 1998

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

Have a follow-up question?

Our Medical A.I. is used by practicing medical doctors at top research institutions around the world. Ask any follow up question and get world-class guideline-backed answers instantly.