What is the duration of stability of Botulinum toxin (Botox) in a refrigeration unit after reconstitution?

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Last updated: April 14, 2025View editorial policy

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From the FDA Drug Label

Store unused reconstituted JEUVEAU refrigerated between 2° to 8°C (36°F to 46°F) in the original carton to protect from light for up to 24 hours until time of use. Discard 24 hours after reconstitution.

Botox can last up to 24 hours when reconstituted and stored in a refrigeration unit at 2° to 8°C (36°F to 46°F) 1.

  • Key points:
    • Store reconstituted Botox in the original carton to protect from light.
    • Do not freeze reconstituted Botox.
    • Discard unused portion after reconstitution and any remaining after 24 hours.

From the Research

Reconstituted Botox can last for at least 4 weeks when stored in a refrigerator at 2-8°C (36-46°F), according to the most recent and highest quality study available 2. The storage time of reconstituted Botox is a critical factor in its efficacy and safety. While most manufacturers and clinical guidelines recommend using reconstituted Botox within 24 hours, some studies suggest that it remains potent for up to 2 weeks under proper refrigeration 3, 4. However, the most recent and highest quality study available, published in 2015, concludes that a vial of toxin reconstituted appropriately can be refrigerated or refrozen for at least 4 weeks before injection without significant risk for contamination or decreased effectiveness 2. Some key points to consider when storing reconstituted Botox include:

  • Using sterile technique during reconstitution to minimize the risk of contamination
  • Storing the product in its original container away from light
  • Clearly labeling the product with the reconstitution date and time
  • Avoiding freezing reconstituted Botox, as it can damage the toxin molecule and reduce effectiveness It's also important to note that the preservative-free nature of Botox makes it susceptible to contamination once reconstituted with saline. Therefore, practitioners should follow their specific product's package insert guidelines and take necessary precautions to ensure the safe and effective use of reconstituted Botox.

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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