Does L-theanine supplement improve testosterone levels in males with low testosterone?

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Last updated: January 21, 2026View editorial policy

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L-Theanine Does Not Improve Testosterone Levels

There is no evidence that L-theanine supplementation improves testosterone levels in males with low testosterone. The provided clinical guidelines and research evidence do not mention L-theanine as a treatment option for hypogonadism, and it is not included in any evidence-based recommendations for testosterone management.

Evidence-Based Approaches for Low Testosterone

Lifestyle Modifications Should Be First-Line

  • Weight loss through low-calorie diets can reverse obesity-associated secondary hypogonadism by improving testosterone levels and normalizing gonadotropins, though increases are modest (1-2 nmol/L) 1
  • Physical activity shows similar benefits, with results correlating to exercise duration and weight loss 1
  • Exercise training enhances lean body mass, aerobic fitness, and strength in aging men, and men who are physically able should be encouraged to exercise 2
  • Adequate sleep, exercise, and weight loss in patients with obesity are imperative before implementing any nutraceutical agents 3

Limited Evidence for Nutraceutical Supplements

Among the supplements studied for testosterone enhancement, only a select few have shown any promise in well-controlled randomized trials 3:

  • Zinc - May improve testosterone in deficient individuals 3
  • Vitamin D - Only beneficial in cases of hypovitaminosis D 3
  • L-arginine - Some evidence for testosterone improvement 3
  • Mucuna and ashwagandha - Limited positive data 3

However, the effects of nonpharmacologic interventions on testosterone levels are modest and do not directly translate into clinical benefits 3.

Most Supplements Lack Evidence

  • A systematic review of popular testosterone supplements found that only 24.8% had data showing an increase in testosterone, while 10.1% actually showed a decrease, and 61.5% had no data at all 4
  • 90% of "testosterone booster" supplements claimed to boost testosterone, but these claims were not supported by the academic literature 4
  • Many supplements contained supra-therapeutic doses of vitamins and minerals, occasionally exceeding the FDA upper tolerable intake level 4
  • No whole supplement products have published randomized controlled trial evidence 5

When Testosterone Replacement Therapy Is Indicated

Diagnostic Criteria

  • Two separate fasting morning testosterone measurements below 300 ng/dL are required for diagnosis, along with hypogonadal symptoms including decreased libido, erectile dysfunction, decreased physical stamina, depressed mood, and fatigue 6, 1
  • Testosterone levels above 350 ng/dL typically do not require replacement therapy 6
  • Testosterone levels below 230 ng/dL usually benefit from testosterone replacement therapy in symptomatic men 6

Treatment Options

If testosterone replacement is indicated, intramuscular or transdermal testosterone therapy results in small improvements in sexual functioning and quality of life 1:

  • Moderate-certainty evidence shows small improvement in global sexual function 1
  • Low-certainty evidence shows small improvement in quality of life 1
  • Little to no effect on physical functioning, depressive symptoms, or cognition 1

Critical Pitfall to Avoid

Patients should be informed that over-the-counter "testosterone booster" supplements, including L-theanine, lack evidence to support their claims 5, 4. Instead of pursuing unproven supplements, focus on:

  1. Confirming true hypogonadism with proper diagnostic testing 6
  2. Implementing lifestyle modifications (weight loss, exercise, adequate sleep) 1, 3
  3. Considering prescription testosterone replacement therapy only when appropriately indicated 1

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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