What are the potential interactions between proton pump inhibitors (PPIs) and trastuzumab in patients with HER2-positive breast cancer?

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Last updated: January 21, 2026View editorial policy

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No Clinically Significant Drug-Drug Interaction Between PPIs and Trastuzumab

There is no established pharmacokinetic or pharmacodynamic interaction between proton pump inhibitors (PPIs) and trastuzumab that affects treatment efficacy or safety. The primary concern with trastuzumab therapy is cardiac toxicity monitoring, not PPI co-administration.

Key Clinical Considerations

Cardiac Monitoring Remains the Priority

  • Cardiac toxicity is the critical safety concern with trastuzumab, with a 4% risk when used as monotherapy and up to 27% when combined with anthracyclines 1.
  • Left ventricular ejection fraction (LVEF) should be evaluated prior to and during treatment, with FDA recommendations for assessment every 3 months during therapy 2.
  • The NCCN guidelines emphasize that increased cardiac toxicity has been consistently observed in patients treated with trastuzumab, particularly when combined with anthracycline and taxane-based regimens 2.

No PPI-Related Treatment Modifications Required

  • Continue trastuzumab therapy without dose adjustment or interruption in patients taking PPIs, as no interaction affecting drug efficacy or safety has been documented in major guidelines 2.
  • The extensive NCCN guidelines for HER2-positive breast cancer management do not identify PPIs as contraindicated medications or drugs requiring special monitoring when used with trastuzumab 2.

Diagnostic Testing Caveat: Biotin Interference

Pre-Treatment HER2 Testing Considerations

  • The only supplement-related concern involves biotin interference with HER2 diagnostic assays, not trastuzumab treatment itself 1.
  • Patients should discontinue biotin supplements at least 72 hours (preferably 7 days for high doses) before HER2 testing to avoid false results in streptavidin-biotin immunoassays 1.
  • Once HER2-positive status is properly confirmed using standardized validated assays (IHC 3+ or FISH amplification >2.0), do not withhold trastuzumab due to any supplement use, including biotin, as early treatment provides maximum survival benefit 1.

Treatment Efficacy Evidence

Established Survival Benefits

  • Trastuzumab consistently demonstrates significant mortality reduction, with an average absolute reduction in 10-year breast cancer mortality by 6.4% (95% CI, 4.9-7.8; P<0.0001) and reduction in all-cause mortality by 6.5% (95% CI, 5.0-8.0; P<0.0001) 2.
  • The benefits of trastuzumab are independent of ER status and remain consistent across multiple large randomized trials 2.

Optimal Treatment Duration

  • Up to 1 year of HER2-targeted therapy with trastuzumab is recommended based on the protocol design of randomized trials establishing trastuzumab benefits 2.
  • For metastatic/stage IV disease, continue HER2-targeted therapy until progression or unacceptable toxicity 2.

Common Clinical Pitfalls to Avoid

  • Do not discontinue or delay trastuzumab therapy due to concomitant PPI use—no evidence supports this practice 2.
  • Do not confuse biotin's diagnostic interference (which affects pre-treatment HER2 testing) with treatment interactions—biotin does not compromise trastuzumab efficacy once therapy is initiated 1.
  • Avoid focusing on non-existent drug interactions when the real monitoring priority is cardiac function assessment 2, 1.

References

Guideline

Biotin and Trastuzumab Interaction: Diagnostic Considerations

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2026

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

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Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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