Is an estradiol level of 200 pg/mL within the normal range for a woman undergoing fertility treatment?

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Last updated: January 24, 2026View editorial policy

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Estradiol Level of 200 pg/mL in Fertility Treatment

An estradiol level of 200 pg/mL is within the normal range for a woman undergoing fertility treatment and generally indicates appropriate ovarian response to stimulation. 1, 2

Normal Reference Ranges

  • Premenopausal women have a follicular phase estradiol range of 51-601 pg/mL, making 200 pg/mL well within normal limits 1
  • Using highly accurate LC-MS/MS methodology, the early follicular phase reference interval is 31-771 pmol/L (approximately 8-210 pg/mL), and the late follicular phase extends to 104-1742 pmol/L (approximately 28-475 pg/mL) 2
  • Estradiol levels below 20 pg/mL warrant investigation for functional hypothalamic amenorrhea or premature ovarian insufficiency, but 200 pg/mL is nearly 10 times this threshold 1, 3

Context in Fertility Treatment

The clinical significance depends on the specific fertility protocol and cycle timing:

  • In human menopausal gonadotropin-intrauterine insemination (hMG-IUI) cycles, birth rates increase progressively with estradiol levels, rising from 3.6% when E2 <500 pg/mL to 19.6% when E2 ≥2,500 pg/mL 4
  • Estradiol correlates with birth rate (r = 0.160, P <0.001) and the number of mature follicles ≥12 mm in ovarian stimulation cycles 4
  • A level of 200 pg/mL suggests mild to moderate ovarian response, which may be appropriate depending on the treatment goal and protocol 4

Important Caveats

Estradiol levels must be interpreted with cycle timing and clinical context:

  • Estradiol should not be interpreted in isolation without knowing cycle day and correlation with menstrual history 1
  • The number of follicles ≥12 mm is more predictive of pregnancy outcomes than estradiol alone in stimulated cycles 4
  • Repeat testing with FSH and LH at specific cycle days (day 3-6) may be necessary to properly assess ovarian reserve and function 1

For women on specific fertility protocols, different thresholds apply:

  • Women receiving GnRH agonists with aromatase inhibitors for breast cancer should have estradiol suppressed to postmenopausal ranges (<7 pg/mL), which is a completely different clinical scenario 3
  • In pubertal induction protocols for cancer survivors, estradiol levels are monitored to guide dose escalation over 24 months, with target levels varying by stage of treatment 5

References

Guideline

Estrogen Levels in Premenopausal Women

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2026

Guideline

Estradiol Levels Requiring Further Diagnostic Workup

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2026

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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