How is urine collected for a Urinalysis (UA) in a feline?

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Last updated: April 16, 2025View editorial policy

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From the Guidelines

Urine for a urinalysis (UA) in cats is best collected via cystocentesis, which involves using a needle and syringe to collect urine directly from the bladder. This procedure should be performed by a veterinarian or trained veterinary technician. The cat is typically gently restrained while the practitioner palpates the bladder, cleans the collection site with antiseptic, and inserts a small needle through the abdominal wall into the bladder to withdraw urine. Cystocentesis is preferred because it provides the cleanest sample without contamination from the lower urinary tract, which is crucial for accurate test results, particularly when bacterial culture is needed, as it prevents contamination with normal bacteria from the lower urinary tract 1.

Some key points to consider when collecting urine for a UA in cats include:

  • The use of a sterile needle and syringe to minimize contamination
  • Gentle restraint of the cat to prevent injury or stress
  • Palpation of the bladder to ensure accurate needle placement
  • Cleaning of the collection site with antiseptic to reduce the risk of infection
  • The potential for mild discomfort during needle insertion, which is generally well-tolerated by most cats

Alternative collection methods, such as catheterization or free catch/voided collection, may introduce contaminants that can affect test results, and are therefore not recommended when a clean sample is required 1. Overall, cystocentesis is the preferred method for collecting urine for a UA in cats due to its ability to provide a clean and accurate sample.

From the Research

Urine Collection Methods for UA in Cats

  • Cystocentesis is a preferred method for collecting urine samples from cats, as it provides a more reliable and accurate diagnosis of urinary tract infections 2, 3.
  • This method involves inserting a needle into the bladder to collect urine, which helps to minimize contamination and provide a more accurate culture result.
  • Other methods, such as catheterization and voiding, can lead to contamination and inaccurate culture results, making it difficult to interpret the results 2, 4, 5.
  • Cystocentesis has been shown to be a safe and effective method for collecting urine samples from cats, with few significant side effects 3.
  • In some cases, cystocentesis may be used as a therapeutic procedure to relieve urinary obstruction and provide temporary relief until the underlying issue can be addressed 3, 6.

References

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Research

Cystocentesis. Diagnostic and therapeutic considerations.

The Veterinary clinics of North America. Small animal practice, 1996

Research

Comparison of collection techniques for quantitative urine culture in dogs.

Journal of the American Veterinary Medical Association, 1978

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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