I'm concerned about testicular shrinkage, with normal Follicle-Stimulating Hormone (FSH) levels, sperm count, and hormone levels, but ultrasound scans (USS) show a potential decrease in testicular volume over time, what could be the cause?

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Testicular Volume Concerns with Normal FSH and Sperm Parameters

Direct Assessment

Your ultrasound measurements are likely incorrect due to technical measurement error, and you should request a repeat scrotal ultrasound with explicit attention to proper measurement technique using the Lambert formula (Length × Width × Height × 0.71) to confirm actual testicular volume. 1

The reported volumes are mathematically inconsistent and suggest caliper placement errors during ultrasound, which commonly leads to severely underestimated and unreliable volume calculations. 1 True biological change in testicular size over 4 weeks is extremely unlikely in adults unless acute pathology is present. 1

Why Measurement Error Is Most Likely

  • Technical factors: High-frequency probes (>10 MHz) must be used to maximize resolution and accurate caliper placement, and measurements should include three perpendicular dimensions (length, width, height) on axial slices. 1

  • Formula matters: The traditional ellipsoid formula (0.52 coefficient) systematically underestimates testicular volume by 20-30% and should never be used for clinical decision-making, as it may lead to inappropriate classification of testicular atrophy. 1 The Lambert formula (0.71 coefficient) provides the most accurate estimates. 1

  • Inter-scan variability: The same sonographer should perform serial measurements when possible, or measurements should be remeasured on previous scans by the current operator to minimize variability. 1

Clinical Context Supporting Measurement Error

  • Normal fertility parameters: Your sperm count of [VALUE]/ml and normal hormone levels in [DATE] make true severe testicular atrophy extremely unlikely. 1 If you have normal fertility, no history of cryptorchidism, and normal secondary sexual characteristics, the likelihood of true severe testicular atrophy is low. 1

  • FSH interpretation: While your FSH values fall within the laboratory reference range, FSH levels >7.6 IU/L indicate impaired but not absent spermatogenesis. 2 Men with FSH >7.5 IU/L have a 5-13 fold higher risk of abnormal sperm concentration compared to men with FSH <2.8 IU/L. 2 However, this indicates reduced testicular reserve rather than progressive atrophy. 1

What You Should Do Now

Immediate Steps:

  • Request repeat ultrasound: Explicitly ask for proper measurement technique using the Lambert formula (0.71 coefficient) with high-frequency probes (>10 MHz). 1 Compare measurements to the contralateral testis, as size discrepancy greater than 2 ml or 20% warrants further evaluation to exclude pathology. 1

  • Obtain complete hormonal panel: Measure FSH, LH, total testosterone, and consider SHBG to calculate free testosterone, as the pattern of gonadotropins helps distinguish primary testicular dysfunction from secondary causes. 1, 3

  • Repeat semen analysis: Obtain at least two semen analyses separated by 2-3 months, as single analyses can be misleading due to natural variability. 1, 3 The assessment of combined ejaculate parameters (concentration, motility, morphology, total count) is more predictive of testicular function than any single parameter. 4

If Repeat Ultrasound Confirms Small Volume (<12 ml):

  • Physical examination: Check for varicocele presence (palpable dilated veins in scrotum), testicular consistency, epididymal abnormalities, and vas deferens patency. 1, 3 Varicocele can cause small testicles with normal hormones, and correction can improve both testicular size and fertility. 3

  • History assessment: Obtain detailed history focusing on cryptorchidism (undescended testicles at birth), infertility concerns, family history of testicular cancer, prior testicular pathology, and any medication use (especially anabolic steroids, opioids, or corticosteroids). 1, 4

  • Consider genetic testing: Karyotype testing is strongly recommended if semen analysis shows severe oligospermia (<5 million sperm/ml) or azoospermia, as chromosomal abnormalities occur in 10% of these patients. 1, 4

Understanding the 12 ml Threshold

  • Clinical significance: Testicular volumes less than 12 ml are definitively considered atrophic and associated with impaired spermatogenesis and increased risk of intratubular germ cell neoplasia (TIN). 1, 3

  • Cancer risk stratification: In men under 30-40 years with testicular volume <12 ml, there is a >34% risk of intratubular germ cell neoplasia in the contralateral testis if testicular cancer is present. 1 History of cryptorchidism substantially increases cancer risk and mandates closer surveillance. 1, 5

  • Fertility correlation: Testicular volume strongly correlates with total sperm count and sperm concentration, with volumes of 15-18 ml associated with normal spermatogenesis and adequate fertility potential. 1

High-Risk Scenarios Requiring Urology Referral

  • Palpable testicular mass develops 1
  • Rapid testicular atrophy occurs 1
  • Severe oligospermia (<5 million/ml) develops 1
  • Age <30 years with confirmed volume <12 ml and history of cryptorchidism 1
  • Size discrepancy between testes >2 ml or 20% 1

Monitoring and Prevention

  • Testicular self-examination: Learn and perform monthly testicular self-examination given potential increased cancer risk with smaller volumes. 1, 4

  • Avoid gonadotoxic exposures: Never use exogenous testosterone or anabolic steroids, as these will completely suppress spermatogenesis through negative feedback, causing azoospermia that can take months to years to recover. 1, 4

  • Serial monitoring: Repeat semen analysis every 6-12 months to detect early decline in sperm parameters if confirmed small testicular volume. 1

Common Pitfalls to Avoid

  • Don't panic over single measurements: Technical ultrasound errors are extremely common and can lead to falsely alarming results. 1

  • Don't treat subclinical varicoceles: Only palpable varicoceles improve fertility outcomes after repair; subclinical varicoceles found only on ultrasound should not be treated. 6, 4

  • Don't start testosterone therapy prematurely: If fertility is desired, testosterone replacement will completely suppress remaining spermatogenesis. 4 Human chorionic gonadotropin (hCG) injections are first-line treatment for restoring testosterone production and spermatogenesis in men with secondary hypogonadism. 4

References

Guideline

Testicular Size and Volume Measurement

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2026

Guideline

Clinical Evaluation of Small Testicles with Normal Hormone Levels

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Guideline

Sperm Production in Atrophied Testicles

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2026

Research

The undescended testis. Hormonal and surgical management.

The Surgical clinics of North America, 1988

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

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Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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