What laboratory tests are recommended for alopecia?

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From the Guidelines

Laboratory tests are not recommended for most cases of alopecia areata.

Recommended Tests

When the diagnosis is in doubt, the following tests may be considered:

  • Fungal culture
  • Skin biopsy
  • Serology for lupus erythematosus
  • Serology for syphilis 1 It is not recommended to routinely test for iron status, as there is no evidence to support a treatment response to iron replacement therapy in patients with alopecia areata 1.

Rationale

The British Association of Dermatologists' guidelines for the management of alopecia areata suggest that investigations are unnecessary in most cases, and that the increased frequency of autoimmune disease in patients with alopecia areata is probably insufficient to justify routine screening 1.

Important Considerations

The management of alopecia areata should take into account the individual's psychological and social well-being, as well as their perceptions of body image and self-esteem 1.

From the Research

Laboratory Tests for Alopecia

The following laboratory tests are recommended for diagnosing alopecia:

  • Complete blood count (CBC) 2
  • Ferritin levels 3, 2
  • Thyroid stimulating hormone (TSH) levels 3, 2
  • Vitamin D 25OH levels 2
  • Vitamin B12 levels 3
  • Hemoglobin levels 3
  • Hair pluck and scalp biopsy may also be required in some cases 4, 2
  • Iron metabolism tests, such as serum iron and total iron-binding capacity, may be useful in some patients 5
  • Hormone tests, such as sex hormone binding globulin (SHBG), total testosterone, and dihydroepiandrostendione (DHEAS), may be considered in patients with female pattern hair loss 5

Specific Tests for Different Types of Alopecia

  • For telogen effluvium, ferritin, hemoglobin, and vitamin B12 levels may be significantly lower, and the number of patients with vitamin D, ferritin, hemoglobin, and vitamin B12 deficiencies may be significantly higher 3
  • For androgenetic alopecia, hormone tests, such as sex hormone binding globulin (SHBG), total testosterone, and dihydroepiandrostendione (DHEAS), may be considered 5
  • For alopecia areata, laboratory tests may not be as useful, but a complete blood count, ferritin, thyroid stimulating hormone, and vitamin D 25OH may still be ordered 2

References

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Research

How to diagnose hair loss.

Dermatologic clinics, 2013

Research

Alopecia: diagnosis and management.

American family physician, 1995

Research

Is hormone testing worthwhile in patients with female pattern hair loss?

Polski merkuriusz lekarski : organ Polskiego Towarzystwa Lekarskiego, 2020

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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