Reconstitution Calculation for CJC-1295
To achieve a concentration suitable for 100-200 mcg dosing from 12.87mg of CJC-1295, add approximately 6.4 mL of sterile water to create a 2 mg/mL solution, allowing you to draw 0.05-0.10 mL (50-100 units on an insulin syringe) per dose.
Mathematical Approach
Converting Your Available Drug Amount
- You have 12.87 mg of CJC-1295 powder
- Target administration: 100-200 mcg per dose
- Convert to same units: 12.87 mg = 12,870 mcg
Determining Optimal Concentration
- A practical concentration is 2 mg/mL (2,000 mcg/mL)
- This allows precise measurement with standard insulin syringes
- Calculation: 12.87 mg ÷ 2 mg/mL = 6.435 mL of sterile water
Dosing After Reconstitution
- For 100 mcg dose: Draw 0.05 mL (50 units on insulin syringe)
- For 200 mcg dose: Draw 0.10 mL (100 units on insulin syringe)
- This concentration provides 64 doses at 200 mcg each
Alternative Concentration Options
More Concentrated Solution (1 mg/mL)
- Add 12.87 mL sterile water for 1 mg/mL concentration
- For 100 mcg: Draw 0.10 mL (100 units)
- For 200 mcg: Draw 0.20 mL (200 units)
- Advantage: Smaller injection volumes
- Disadvantage: Requires more precise measurement
Less Concentrated Solution (0.5 mg/mL)
- Add 25.74 mL sterile water for 0.5 mg/mL concentration
- For 100 mcg: Draw 0.20 mL (200 units)
- For 200 mcg: Draw 0.40 mL (400 units)
- Advantage: Easier to measure accurately
- Disadvantage: Larger injection volumes
Critical Safety Considerations
Reconstitution Technique
- Use bacteriostatic water for injection if planning multiple doses over several weeks
- Inject sterile water slowly down the side of the vial to minimize foaming
- Gently swirl (never shake) to dissolve the powder completely
- Allow to sit for 2-3 minutes if cloudiness persists
Storage and Stability
- Store reconstituted solution refrigerated at 2-8°C (36-46°F)
- Protect from light using amber vials or aluminum foil wrap
- Use within 28 days if using bacteriostatic water
- Use within 72 hours if using sterile water without preservative
- Discard if solution becomes cloudy, discolored, or contains particles
Administration Precautions
- CJC-1295 is typically administered subcutaneously 1, 2
- Clinical studies used doses of 30-60 mcg/kg, with your target range (100-200 mcg) falling within therapeutic parameters for an average adult 2
- Research demonstrates sustained GH elevation for 6+ days after single injection, suggesting infrequent dosing schedules 2
Verification of Calculations
Double-Check Formula
- Desired concentration (mg/mL) = Total drug amount (mg) ÷ Volume of diluent (mL)
- 2 mg/mL = 12.87 mg ÷ 6.435 mL ✓
- Each 0.1 mL contains: 2 mg/mL × 0.1 mL = 0.2 mg = 200 mcg ✓
Common Measurement Errors to Avoid
- Confusing mg with mcg (1 mg = 1,000 mcg)
- Using non-sterile water or inappropriate diluents
- Failing to account for overfill in vials when calculating total doses
- Not verifying syringe calibration (insulin syringes measure in units where 100 units = 1 mL)