What is the appropriate workup for a patient presenting to the Emergency Department (ED) with anisocoria?

Medical Advisory BoardAll articles are reviewed for accuracy by our Medical Advisory Board
Educational purpose only • Exercise caution as content is pending human review
Article Review Status
Submitted
Under Review
Approved

Last updated: January 31, 2026View editorial policy

Personalize

Help us tailor your experience

Which best describes you? Your choice helps us use language that's most understandable for you.

Anisocoria Workup in the Emergency Department

In the ED, immediately determine which pupil is abnormal by examining pupillary responses in both bright and dim lighting, then assess for pupil-involving third nerve palsy (dilated pupil with ptosis and/or ophthalmoplegia), which requires urgent neuroimaging to rule out posterior communicating artery aneurysm—a neurosurgical emergency. 1, 2

Initial Critical Assessment

The first priority is identifying life-threatening causes:

  • Check pupil size in bright AND dim lighting to determine which pupil is abnormal 1, 2
  • Examine for ptosis and extraocular movement limitations immediately 1, 2, 3
  • Assess for associated neurological deficits including headache, altered mental status, or focal findings 2

Red Flags Requiring Urgent Neuroimaging

Obtain immediate MRI with gadolinium and MRA or CTA for: 1, 2, 3

  • Pupil-involving third nerve palsy (dilated pupil with ptosis and/or ophthalmoplegia) 1, 2
  • Anisocoria with headache or altered mental status 2
  • Anisocoria following head trauma 2
  • Any new neurological deficits accompanying anisocoria 2

Critical Nuance on Third Nerve Palsy

  • Complete ptosis with complete ophthalmoplegia but NORMAL pupil (classic pupil-sparing third nerve palsy) is almost always microvascular from diabetes, hypertension, or hyperlipidemia and does NOT require emergent imaging 1, 3
  • However, if there is partial extraocular muscle involvement OR incomplete ptosis, even with a normal pupil, do NOT assume microvascular etiology—obtain neuroimaging 1

Systematic Diagnostic Algorithm

Step 1: Rule Out Pharmacologic Mydriasis

Before ordering expensive imaging, consider recent medication exposure: 4, 5, 6, 7

  • Nebulized ipratropium bromide can leak from face masks and cause unilateral mydriasis 4, 7
  • Topical anticholinergics, antihistamines, or tropane alkaloids can cause pharmacologic mydriasis 1
  • Pilocarpine 1% testing: Pharmacologically dilated pupils will NOT constrict with pilocarpine 1%, while third nerve palsy pupils will constrict 1, 4, 6

Step 2: Assess for Acute Angle-Closure Crisis

If the enlarged pupil is mid-dilated, oval, or asymmetric with associated symptoms: 1

  • Perform gonioscopy and measure intraocular pressure immediately 1
  • Initiate IOP-lowering therapy if angle-closure confirmed 1
  • The fellow eye requires urgent prophylactic laser iridotomy 1

Step 3: Perform Slit-Lamp Examination

Essential findings to document: 1, 3

  • Pupil size, shape, and reactivity 1
  • Irregular pupils suggest traumatic sphincter damage, iritis, or congenital abnormality 1
  • Check for relative afferent pupillary defect (RAPD), which should prompt search for compressive optic neuropathy or retinal pathology 1

Step 4: External Examination

Assess for: 1, 3

  • Ptosis and levator function 1, 3
  • Eyelid retraction, proptosis, or globe retraction 1
  • Head position abnormalities 1

Benign Causes (Diagnosis of Exclusion)

Physiologic anisocoria can be diagnosed when: 1, 2

  • Difference is typically <1 mm 1, 2
  • Pupils react normally to light 1, 2
  • No ptosis or other abnormalities present 1, 2
  • Anisocoria remains constant in different lighting conditions 2

Common Pitfalls to Avoid

  • Do NOT assume microvascular third nerve palsy without complete ptosis, complete ophthalmoplegia, AND normal pupil—partial presentations require imaging 1
  • Do NOT order MRI before checking for metallic intraocular foreign bodies—undiagnosed metallic objects can move during MRI causing serious ocular injury 8
  • Do NOT skip pilocarpine testing in patients recently exposed to nebulized medications—this simple test prevents unnecessary radiation exposure 4, 7
  • Do NOT attribute large RAPD to amblyopia alone—always search for compressive optic neuropathy or retinal abnormality 1

Disposition Considerations

Neurosurgical consultation required if aneurysm identified 1, 2

Neurology or neurosurgery referral for cavernous sinus lesions (multiple cranial nerve palsies including III, IV, VI, V1) 1, 2

Pathological causes require follow-up based on underlying etiology and specialist recommendations 1

References

Guideline

Diagnostic Approach to Anisocoria

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2026

Guideline

Anisocoria Causes and Diagnostic Approach

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Guideline

Evaluation of Spontaneous Ptosis

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2026

Research

Approach to anisocoria in the emergency department.

The American journal of emergency medicine, 2023

Research

Pharmacological testing of anisocoria.

Expert opinion on pharmacotherapy, 2005

Research

Reversible Mydriasis of Sudden Onset: Think Before You Act.

The Journal of emergency medicine, 2021

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

Have a follow-up question?

Our Medical A.I. is used by practicing medical doctors at top research institutions around the world. Ask any follow up question and get world-class guideline-backed answers instantly.