What is the definition of Pyrexia of Unknown Origin (PUO) in pediatric patients?

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Last updated: January 31, 2026View editorial policy

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Definition of Pyrexia of Unknown Origin (PUO) in Children

In pediatric patients, PUO is defined as fever ≥38.3°C (101°F) persisting for at least 3 weeks with no diagnosis despite 3 outpatient visits or 3 inpatient days of investigation. 1, 2

Core Diagnostic Criteria

The definition requires three essential components:

  • Temperature threshold: Fever must be ≥38.3°C (101°F), though some pediatricians use >38°C as their cutoff in practice 1, 3
  • Duration requirement: Fever must persist for at least 3 weeks, distinguishing PUO from acute fever without source (FWS), which is an acute illness with no apparent source after initial evaluation 4
  • Investigation requirement: No diagnosis should be apparent despite 3 outpatient visits or 3 inpatient days of investigation 1, 2

Important Distinction: Prolonged FUO vs. Classical PUO

Prolonged FUO represents a separate clinical entity in children, defined as documented fever lasting more than 7 to 10 days with no apparent source and no diagnosis after 1 week of clinical investigations. 5 This shorter timeframe (7-10 days vs. 3 weeks) reflects the practical reality that children with persistent unexplained fever often require earlier intervention than the classical 3-week definition would suggest.

Age-Specific Considerations

  • Neonates and infants (<3 months): Have the highest risk for invasive bacterial infections, with 8-13% having bacterial infections (predominantly urinary tract infections), and infants <28 days are at highest risk 1
  • Young children (>3 months to 36 months): Require exclusion of congenital or cardiac disease in those who are febrile and ill-appearing, and 25% may show pneumonia on chest radiograph despite no obvious respiratory source 1

Classification System

PUO should be classified into four subcategories to guide workup, as etiology distribution varies significantly:

  • Classical PUO (community-acquired)
  • Nosocomial PUO
  • Neutropenic PUO (defined as single oral temperature ≥38.3°C OR ≥38.0°C sustained over at least 1 hour in neutropenic patients) 2, 4
  • HIV-related PUO 2, 4

Critical Pitfall to Avoid

Do not confuse PUO with "fever without source" (FWS)—FWS is an acute illness with no apparent source after initial evaluation, while PUO requires prolonged fever >3 weeks with no identifiable source. 4 This distinction is crucial because the diagnostic approach, differential diagnosis, and urgency of intervention differ substantially between these two entities.

Temperature Measurement Standards

  • Central temperature monitoring (pulmonary artery catheter, bladder catheters, or esophageal balloon thermistors) is preferred for accurate diagnosis in inpatient settings 4
  • Oral or rectal temperatures are reliable alternatives when central monitoring is unavailable 4
  • Avoid axillary, tympanic membrane, temporal artery thermometers, or chemical dot thermometers for diagnostic purposes due to unreliability 4

References

Guideline

Diagnostic Approach to Pyrexia of Unknown Origin

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2026

Guideline

Evaluation Algorithm for Pyrexia of Unknown Origin

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Guideline

Diagnostic Approach to Fever of Unknown Origin

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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